How it Works: Real Time PCR :The Scientist [2006-12-01]


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How it Works: Real Time PCR The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for recording, and a computer to control the instrument and record data. Increasingly sophisticated instruments, such as those capable of multiplex experiments, are becoming affordable in academic labs. The light source in the