High-throughput RNAi libraries for mammalian cells are nearing a new maturity with the first wave of virally transfected short hairpin (sh)RNA libraries finally making it into published work. Developers are still making major and frequent improvements, so comparisons are difficult. "Most of the RNAi field is unfortunately anecdotal," says David Sabatini of MIT's Whitehead Institute. "That's a very dangerous thing."
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An RNAi Rogue's Gallery
How to size up the available libraries for high throughput screening in mammalian cells.
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