When it comes to posttranslational modifications, phosphorylation gets most of the attention, but glycosylation is more widespread. Nearly all cell-surface and secreted proteins are adorned with oligosaccharides, which affect cell-cell interactions, immune cell function, and antigenicity. Changes in glycosylation patterns have been linked to cancer, making glycoproteins ripe for biomarker discovery.
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Isolating and detecting glycosylated proteins
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