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tag real time pcr ecology

What Price Ecological restoration?
Paddy Woodworth | Apr 1, 2006 | 9 min read
FEATURERestoring Natural Capital In putting a price tag on endangered species and degraded ecosystems, ecologists and economists have joined forces to formulate a new rationale for environmental issues: restoring natural capital© Erich Schlegel/Dallas Morning News/CorbisBY PADDY WOODWORTHEcological restoration is expensive. The United States government is slated to spend almost $8 billion restoring parts of the Florida Evergla
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Assay for Sickle Cell Anemia Is Repurposed to Diagnose COVID-19
G.B.S.N.P. Varma | Nov 18, 2020 | 5 min read
A CRISPR-based, paper-strip test developed by Indian researchers performs as well as real-time PCR in identifying the presence of SARS-CoV-2 and returns results within an hour.
Eytan Stibbe wears a headset and a blue shirt while surrounded by computers and other equipment on the International Space Station.
Whenever, Wherever: Taking DNA Amplification Outside the Lab
Hannah Thomasy, PhD | Dec 4, 2023 | 10 min read
Recombinase polymerase amplification lets researchers rapidly replicate DNA in the clinic, in the field, or even in the International Space Station.
Green and red fluorescent proteins in a zebrafish outline the animal’s vasculature in red and lymphatic system in green in a fluorescent image. Where the two overlap along the bottom of the animal is yellow.
Serendipity, Happenstance, and Luck: The Making of a Molecular Tool
Shelby Bradford, PhD | Dec 4, 2023 | 10+ min read
The common fluorescent marker GFP traveled a long road to take its popular place in molecular biology today.
Stalking Sharks
Jef Akst | Aug 30, 2012 | 3 min read
Researchers monitor the movement of the Pacific’s largest predators and share the information with the world in real time.
Up to Speed on PCR
Deborah Fitzgerald | Nov 26, 2000 | 7 min read
Real-time PCR Systems Cepheid's Smart Cycler System PCR--a technique so common in today's laboratories that it is easy to forget its revolutionary impact--enables the specific amplification and detection of as little as a single copy of a particular nucleotide sequence. However, PCR has the potential to be used not just for the detection of specific sequences, but also for their quantification, because of the quantitative relationship between the amount of starting target sequence and the amoun
Genotyping with PCR
M. Tevfik Dorak | Jun 1, 2007 | 5 min read
How to choose the right approach
Fluorescence microscopy image of cells expressing fluorescent biosensors. Green and magenta fluorescence is observed outside of the cell nuclei.
Choosing Fluorescent Reagents for Every Live Cell Application
The Scientist and MilliporeSigma | Nov 30, 2022 | 4 min read
Scientists gain unique insights into active biological processes with specific fluorescent probes, dyes, and biosensors.
A Beacon in the Night
Sarah Goforth | May 27, 2001 | 2 min read
The precise molecular recognition event of nucleic acid hybridization is fundamental to molecular biology. Traditionally, probes are tagged with fluorescent or radioactive labels and hybridized to a sample. Unbound probe is removed via a dilution or digestion step. This allows the investigator to quantify the amount of bound probe but simultaneously disrupts the equilibrium state of the hybridization, preventing the use of this technique in real-time or in vivo. Molecular beacons, single-stran
High-Throughput Thermocyclers
Jeremy Peirce | Nov 10, 2002 | 8 min read
Photo: Courtesy of Applied Biosystems The Auto-Lid Dual 384-Well GeneAmp PCR System 9700 from Applied Biosystems. Used to be, researchers used thermocyclers for PCR. But times have changed. Once called PCR machines thermocyclers are now required for a wide range of common applications such as sequencing and genotyping. Murray Anderson, director of core PCR for Applied Biosystems, Foster City Calif., observes, "When we first launched the dual 384-well [thermal cycling] systems, the primar

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