Genetic analyses lay to rest conspiracy theories about death of Belgian King Albert I, who lost his life in a rock climbing accident more than 80 years ago.
July 17, 2001|
The yeast two-hybrid system is one of the most widely used functional genomic tools for studying protein-protein interactions. But transcriptional activators cannot be used as 'bait' proteins in the assay as they can activate the reporter gene, usually used as an indicator of protein interaction, even in the absence of protein interactions. In the July 17 Proceedings of the National Academy of Sciences, Hirst et al. describe a modified two-hybrid assay that is based on transcriptional repression