The largely overlooked resident fungal community plays a critical role in human health and disease.
November 23, 2000|
Two-hybrid assays are great for detecting protein-protein interactions, but they scale as the square of the number of candidate interactions. Screening every possible interaction in yeast would require examining approximately 3.6 x 107 pairs, which is why previous large-scale screens by Uetz et al. and Ito et al. have pooled libraries of constructs. In the November 21 Proceedings of the National Academy of Sciences, Newman et al. find that such approaches can miss a great number of valid interac