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Creative Emulsification
Sabrina Richards | Nov 1, 2012 | 8 min read
Enhancing data collection from emulsion PCR reactions: three case studies
Microarrays at Work
Amy Francis | Jun 11, 2000 | 8 min read
Photo: Jeff MillerTomas A Prolla Editor's Note: To keep up with topical research trends, LabConsumer continues to address various aspects of microarray technology in 2000. "Array of Options" (May 29, 2000) described the features of commercially available systems for arraying, scanning, and analyzing microarrays, and an upcoming issue (Sept. 4, 2000) will profile the growing variety of premade arrays for various applications. To gain further access to this burgeoning field, LabConsumer entered th
Getting The Message With RT-PCR
Deborah Wilkinson | Aug 16, 1998 | 10+ min read
Date: July 20, 1998RT-PCR Kits Reverse transcription followed by the polymerase chain reaction (RT-PCR) has become one of the great "workhorse" techniques of today's labs. It is often used as a method for generating needed reagents, including complementary DNA (cDNA) inserts for cloning, cDNA libraries, and templates for in vitro transcription. None of the other commonly used methods for measuring the steady-state levels of individual RNAs (such as Northern or dot blotting, RNase or S1 nuclease
High-Throughput Thermocyclers
Jeremy Peirce | Nov 10, 2002 | 8 min read
Photo: Courtesy of Applied Biosystems The Auto-Lid Dual 384-Well GeneAmp PCR System 9700 from Applied Biosystems. Used to be, researchers used thermocyclers for PCR. But times have changed. Once called PCR machines thermocyclers are now required for a wide range of common applications such as sequencing and genotyping. Murray Anderson, director of core PCR for Applied Biosystems, Foster City Calif., observes, "When we first launched the dual 384-well [thermal cycling] systems, the primar
Using CRISPR to Edit Genes in Induced Pluripotent Stem Cells
Kelly Rae Chi | Sep 1, 2016 | 8 min read
Tips on how to surmount the challenges of working with CRISPR to manipulate genes in human stems cells to study their function in specific diseases or to correct genetic defects in patient cells.
Taking The Measure Of The Message
Laura De Francesco | Nov 22, 1998 | 9 min read
Date: November 23, 1998Product Comparison Getting a measure of even a single RNA species has never been easy. Every few years, a new technique comes along--Northerns, ribonuclease protection assays, RT-PCR--that makes the task only incrementally easier. But a quantum leap in technology took place just a few years ago with the introduction of cDNA arrays, and suddenly not just one but hundreds to thousands of target nucleic acids can be analyzed simultaneously and precisely. Since then, array te
2006 Life Science Industry Awards
Jeffrey M. Perkel and Ishani Ganguli | Apr 1, 2006 | 10+ min read
FEATURELSIA 2006 Our fourth annual event celebrates excellence in life science product development and services BY JEFFREY M. PERKEL AND ISHANI GANGULI It has been said that scientists stand on the shoulders of giants. Generally that refers to researchers' intellectual forebears, but it also is true of the life science industry - technology giants that provide the scientific community with the tools, equipment and
Top 10 Innovations 2014
The Scientist | Dec 1, 2014 | 10+ min read
The list of the year’s best new products contains both perennial winners and innovative newcomers.
Breaking Up Isn't Hard To Do: A cacophony of sonicators, cell bombs, and grinders
Brent Johnson | Nov 8, 1998 | 10+ min read
Date: November 9, 1998Comparison of Disruptors With names such as cell crushers, grinders, disintegrators, and pulverizers dominating the field, the business of cell disruption is not for the faint of heart. Some of the functions of these machines are so twisted and diabolical that they would make the Marquis de Sade wince. These devices do the dirty work of the research world, ripping and tearing at the fabric of plants and animal tissues with extreme prejudice to both forms of life. Many scie
Kits to Dye For: A Profile of Sequencing Kits for Automated DNA Sequencers
Michael Brush | Nov 9, 1997 | 9 min read
Date: November 10, 1997 Chart 1 In the long series of events inherent in automated DNA sequencing, cranking out DNA labeled with fluorescent tags is, of course, the most important element of a successful procedure. Without properly labeled sequence ladders to analyze, those expensive, automated DNA sequencers have little to do. So to keep them busy, LabConsumer checked out fluorescent automated DNA sequencing kits from eight manufacturers. The kits profiled exploit two methods for labeling se

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