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tag reagents salary emulsion pcr dna sequencing

Molecule of DNA forming inside the test tube equipment.3d rendering,conceptual image.
EvaGreen® Dye: The Swiss Army Knife of qPCR
Biotium | Mar 1, 2024 | 7 min read
A green fluorescent dye with a novel DNA binding mechanism improves signal-to-noise in different DNA amplification assays.
Creative Emulsification
Sabrina Richards | Nov 1, 2012 | 8 min read
Enhancing data collection from emulsion PCR reactions: three case studies
Kits to Dye For: A Profile of Sequencing Kits for Automated DNA Sequencers
Michael Brush | Nov 9, 1997 | 9 min read
Date: November 10, 1997 Chart 1 In the long series of events inherent in automated DNA sequencing, cranking out DNA labeled with fluorescent tags is, of course, the most important element of a successful procedure. Without properly labeled sequence ladders to analyze, those expensive, automated DNA sequencers have little to do. So to keep them busy, LabConsumer checked out fluorescent automated DNA sequencing kits from eight manufacturers. The kits profiled exploit two methods for labeling se
Cycle Sequencing Kits
Michael Brush | Jul 20, 1997 | 10+ min read
When the next time around is always better. Date: July 21, 1997 Comparison Chart DNA sequencing methods have come a long way in 20 years. From the chemical method of Maxam and Gilbert and the dideoxy method of Sanger, DNA sequencing techniques evolved into the "labeling/termination" method that used a modified T7 DNA polymerase such as Sequenase. Propelled by the power and potential of DNA amplification using thermal cycling strategies, earlier DNA sequencing methodologies have increasingly b
Sequencing On Target
Jeffrey M. Perkel | May 1, 2009 | 7 min read
By Jeffrey M. Perkel Sequencing On Target Techniques for pulling out and sequencing selected areas of the genome It's time for a genomics reality check. Despite the constant, glowing coverage of speedy, low-cost next-generation DNA sequencing, whole-genome analysis, and consumer genomics, researchers still have no idea what the vast majority of human genomic DNA does, nor the functional consequence of variations in those sequences. Thus, few researchers
Up to Speed on PCR
Deborah Fitzgerald | Nov 26, 2000 | 7 min read
Real-time PCR Systems Cepheid's Smart Cycler System PCR--a technique so common in today's laboratories that it is easy to forget its revolutionary impact--enables the specific amplification and detection of as little as a single copy of a particular nucleotide sequence. However, PCR has the potential to be used not just for the detection of specific sequences, but also for their quantification, because of the quantitative relationship between the amount of starting target sequence and the amoun
High Fidelity PCR: Enhancing the Accuracy of DNA Amplification
Shane Beck | Jan 4, 1998 | 10 min read
Date: January 5, 1998 Chart 1, Chart 2 n the beginning there was Taq. Actually, there were others before Taq. There were precursory polymerases, such as that from E. coli, that lost their enzymatic activities at elevated temperatures. This shortfall made thermal cycling a time-consuming chore, with the necessity of adding new polymerase after each cycle. Then came the thermostable polymerases such as Taq DNA polymerase, which was isolated from the thermophilic, aerobic bacterium Thermus aquat
RACE to the 5' End
Jeffrey Perkel | Jan 7, 2001 | 2 min read
Invitrogen's Gene Racer Kit Expressed sequence tags are powerful tools for gene expression studies, but when there is simply no substitute for a full-length cDNA, researchers turn to protocols for the Rapid Amplification of cDNA Ends (RACE). RACE techniques couple cDNA synthesis with a method that attaches a "known" sequence to the end of the gene to enable PCR amplification. Knowing the 5' end of a transcript can aid promoter, gene mapping, and alternate exon usage analyses. Unfortunately, 5' R
PCR Based Cloning Kits: Something For Everybody
Laura Defrancesco | Apr 12, 1998 | 10+ min read
Date: April 13, 1998PCR Based Cloning Kits Table The End Table (PDF Format) PCR has found applications in almost every imaginable facet of molecular biology, and for many applications, looking at a band on a gel is not enough. Sequencing, expressing, mutating--all require cloning. And as it happens, cloning strategies that work for other types of DNA fragments don't work at all well, or require inordinate effort, with PCR fragments. For example, the most commonly used cloning strategy requires
Genotyping with PCR
M. Tevfik Dorak | Jun 1, 2007 | 5 min read
How to choose the right approach

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