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Tips on how to surmount the challenges of working with CRISPR to manipulate genes in human stems cells to study their function in specific diseases or to correct genetic defects in patient cells.

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image: In Custody

In Custody

By | April 1, 2015

Expert tips for isolating and culturing cancer stem cells

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image: Picturing Infection

Picturing Infection

By | January 1, 2015

Whole-animal, light-based imaging of infected small mammals


image: Surviving the Ice Age

Surviving the Ice Age

By | September 1, 2014

A beginner’s guide to freezing and thawing pluripotent stem cells


image: Capturing Cancer Cells on the Move

Capturing Cancer Cells on the Move

By | April 1, 2014

Three approaches for isolating and characterizing rare tumor cells circulating in the bloodstream


image: Lights, Camera, Action

Lights, Camera, Action

By | January 1, 2014

A guide for doing in vivo microscopy on neurons in the mammalian brain


image: Seeing Double

Seeing Double

By | November 1, 2013

Combining two imaging techniques integrates molecular specificity with nanometer-scale resolution.  


image: Synapses on Stage

Synapses on Stage

By | November 1, 2013

Light microscopy techniques that spotlight neural connections in the brain


image: Suited to a T

Suited to a T

By | May 1, 2013

Sorting out T-cell functional and phenotypic heterogeneity depends on studying single cells.

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image: Structure by Feel

Structure by Feel

By | April 1, 2013

Applying the sensitive touch of atomic force microscopy to DNA, cells, and proteins

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