Jack Lucentini | Sep 12, 2004 | 6 min read
DELIVERY METHODS:© 2003, Elsevier ScienceSmall interfering RNAs (siRNAs) may be synthesized and then transfected into cells (A), or generated by the RNAase activity of Dicer (B) on short hairpin RNAs (shRNAs) transcribed in vivo (C). Transfection (D) and integration (E) of plasmid DNA containing a selectable marker and a promoter to drive shRNA expression are used to generate a stable cell line. Alternatively, DNA encoding shRNA may be introduced by viral-mediated transduction (D). siRNAs (