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tag decomposition microbiology

Articles Alert
Simon Silver | Jul 8, 1990 | 7 min read
The Scientist has asked a group of experts to comment periodically upon recent articles that they have found noteworthy. Their selections, presented herein every issue, are neither endorsements of content nor the result of systematic searching. Rather, the list represents personal choices of articles the columnists believe the scientific community as a whole may also find interesting. Reprints of any articles cited here may be ordered through The Genuine Article, 3501 Market St., Philadelphia,
A Thousand Points of Light
Bob Sinclair | Oct 15, 2000 | 10+ min read
Not so long ago, researchers had somewhat limited choices for locating and following a particular piece of DNA. A probe could be labeled using radioactivity, by kinasing an end or nick-translating the whole piece. A fragment of interest could be visualized (along with all other DNA and RNA species in the preparation) using ethidium bromide. With sufficient skill and patience an investigator could obtain from these rather crude techniques fairly impressive information, such as the precise 5' end

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