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tag mmr genetics genomics

A Thousand Points of Light
Bob Sinclair | Oct 15, 2000 | 10+ min read
Not so long ago, researchers had somewhat limited choices for locating and following a particular piece of DNA. A probe could be labeled using radioactivity, by kinasing an end or nick-translating the whole piece. A fragment of interest could be visualized (along with all other DNA and RNA species in the preparation) using ethidium bromide. With sufficient skill and patience an investigator could obtain from these rather crude techniques fairly impressive information, such as the precise 5' end

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