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tag digital pcr immunology microbiology

The Search for Persisters
Amanda B. Keener | Aug 11, 2015 | 4 min read
Lyme disease–causing bacteria can outmaneuver antibiotics in vitro and manipulate the mouse immune system.
Bioterrorism Research: New Money, New Anxieties
John Dudley Miller | Apr 6, 2003 | 8 min read
Ned Shaw US scientists have reason to feel both heady and scared. The federal government recently released unprecedented billions of dollars to fund bioterrorism research. Yet, the merits of this sudden shift in focus are being debated, and some worry that the money will be squandered or wasted. "I have been really very upset by the focus on bioterrorism," says Stanley Falkow, professor of microbiology and immunology and of medicine at Stanford University. "Everybody's talking about it, but th
Top 10 Innovations 2013
The Scientist | Dec 1, 2013 | 10+ min read
The Scientist’s annual competition uncovered a bonanza of interesting technologies that made their way onto the market and into labs this year.
2021 life science predictions innovations biotech mrna single-cell sequencing diagnostics
Experts Predict the Hottest Life Science Tech in 2021 and Beyond
Bob Grant | Feb 1, 2021 | 7 min read
Last year’s Top 10 Innovations judges look into their crystal balls.
2020 Top 10 Innovations
The Scientist | Dec 1, 2020 | 10+ min read
From a rapid molecular test for COVID-19 to tools that can characterize the antibodies produced in the plasma of patients recovering from the disease, this year’s winners reflect the research community’s shared focus in a challenging year.
Taking The Measure Of The Message
Laura De Francesco | Nov 22, 1998 | 9 min read
Date: November 23, 1998Product Comparison Getting a measure of even a single RNA species has never been easy. Every few years, a new technique comes along--Northerns, ribonuclease protection assays, RT-PCR--that makes the task only incrementally easier. But a quantum leap in technology took place just a few years ago with the introduction of cDNA arrays, and suddenly not just one but hundreds to thousands of target nucleic acids can be analyzed simultaneously and precisely. Since then, array te
Flow Cytometry On-a-Chip
Jeffrey M. Perkel | Jun 1, 2015 | 7 min read
Novel microfluidic devices give researchers new ways to count and sort single cells.
Get the Basics
Gail Dutton | Nov 10, 2002 | 6 min read
Photo: Courtesy of Michelle Lizott Waniewski  DROP AND GIVE ME 20! Attendees at the 2002 NEB workshops labor through basic training. If you've ever been confused by the jargon of molecular biology, you are not alone. With biotechnology in the news day by day, it is easy to forget that for many of us--even for some readers of The Scientist--the tools, techniques, and concepts that undergird much of this research are foreign. To help keep up, some scientists are queuing up to learn the bas
A River Runs Through It
Bob Klevecz | Jan 18, 1998 | 7 min read
Flow cytometry instruments analyze suspensions of single cells by focusing them hydrodynamically in a columnar stream and causing them to flow past a point or points in the stream where lasers have been focused. As each cell passes through the point of excitation, it scatters the laser light, and if it is appropriately stained, it will also fluoresce. Sensors collect both the scattered light and fluorescence, and based on the timing of the pulses, the collected signals can be identified with an
Assays by the Score
Deborah Fitzgerald | May 27, 2001 | 9 min read
Click to view the PDF file: Bead-based Fluorescent Multiplex Protein Analysis Systems Courtesy of LINCO ResearchLabMAP-based systems use internally dyed fluorescent microspheres to analyze as many as 100 different analytes concurrently. Today's competitive, high-paced research environment has stimulated the development of a host of approaches for rapid, cost-efficient analyses of large numbers of samples. In keeping with this trend, methods for simultaneously analyzing multiple species in a g

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