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tag cleavage plane genetics genomics

bacteria and DNA molecules on a purple background.
Engineering the Microbiome: CRISPR Leads the Way
Mariella Bodemeier Loayza Careaga, PhD | Mar 15, 2024 | 10+ min read
Scientists have genetically modified isolated microbes for decades. Now, using CRISPR, they intend to target entire microbiomes.
Advances in Genome Editing
Catherine Offord | Apr 20, 2016 | 4 min read
Researchers develop a CRISPR-based technique that efficiently corrects point mutations without cleaving DNA.
Preimplantation Genetic Diagnosis: The Next Big Thing?
Ricki Lewis | Nov 12, 2000 | 9 min read
Courtesy of David Hill, ART Reproductive Center Inc.Two separated blastomeres subjected to FISH analysis to check the chromosomes. In early October, preimplantation genetic diagnosis (PGD) made headlines when a Colorado couple used assisted reproductive technology (ART) to have a baby named Adam, whose umbilical cord stem cells could cure his six-year-old sister Molly's Fanconi anemia.1 When Adam Nash was a ball of blastomere cells, researchers at the Reproductive Genetics Institute at Illinois
2017 Top 10 Innovations
The Scientist | Dec 1, 2017 | 10+ min read
From single-cell analysis to whole-genome sequencing, this year's best new products shine on many levels.
Reductio Ad Amino Acid
Bob Sinclair | Feb 1, 1999 | 10+ min read
Date: February 1, 1999Fusion/Tag Proteases TableProteolytic Enzymes TableTable 3Table 4 A proteome analysis aims to characterize all proteins expressed by an organism or tissue. The next step will be to correlate a protein profile with the appropriate genome, and beyond that researchers will want to understand the correlations between levels of proteins, co- and post-translational modifications, and cell or tissue activity. Many of the technologies that are necessary to realize this goal are de
Cookbook For Eukaryotic Protein Expression: Yeast, Insect, and Plant Expression Systems
Christopher Smith | Nov 8, 1998 | 10+ min read
Date: November 9, 1998Baculovirus Expression Vectors In the recent past, efforts to elucidate the relationship between protein structure and biological function have intensified. Of particular interest is an understanding of the elements of sequence and structure that mediate specific functions. Often the protein of interest is in low abundance in its natural source and can be difficult to purify and/or unstable--subject to proteolytic cleavage or unfolding/non specific refolding during exhaust
Enhancer and Promoter Atlases
Ashley P. Taylor | Mar 26, 2014 | 4 min read
Consortium annotates the human genome with cell type-specific information about transcription start sites, active enhancers, and their expression throughout the body.
Cool Cloning
Karen Hopkin | Jul 1, 2008 | 7 min read
Lynn Cooley figured she'd study sea creatures, then decided to revolutionize genetics instead.
Deviations From The Norm: Systems For Mutation Detection Reveal Hidden Potentials
Kailash Gupta | Jul 18, 1999 | 10+ min read
Date: July 19, 1999Mutation Detection Systems and Methods Affymetrix's p53 chip Researchers leading the Human Genome Project (HGP) originally envisioned completion of the entire genome sequence (approximately 3 billion base pairs) by the year 2005. Recently the arena of human genome sequencing has seen a lot of heat generated by the entry of both commercial entrepreneurs and public consortia. Celera, a company formed by highly skilled and competent commercial organizations (TIGR and Perkin-Elm
Yeast: An Attractive, Yet Simple Model
Gregory Smutzer | Sep 16, 2001 | 9 min read
Yeast possesses many characteristics that make it especially useful as a model system in the laboratory, including an entirely sequenced genome. Recently, a number of researchers published studies detailing the transition from genome sequencing to functional genomics. Notably, these scientists have developed new high-throughput approaches to the characterization of large numbers of yeast genes. In aggregate, these studies make yeast one of the most well-characterized eukaryotic organisms known.

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