The Scientist Staff | Feb 5, 1995 | 3 min read
The Ni-NTA spin columns provide purification of 6xHis-tagged proteins from small-scale expression cultures. Available in two formats--separately without reagents or as a complete kit--they can be used in experimental situations requiring small-scale expression, functional screening, and rapid purification of engineered and recombinant proteins. Each can purify up to 100 fg of 6xHis tagged protein under native or denaturing conditions. According to the manufacturer, this procedure allows 12 mini