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tag immunofluorescence developmental biology

Playing Protein Hide and Seek
Mignon Fogarty | Apr 11, 2004 | 6 min read
LIGHTING THEIR LOCATIONS:© 2002 Cold Spring Harbor PressImmunolocalization of epitope-tagged proteins. (A-E) represent cells containing HAT-tagged proteins stained with the DNA dye, DAPI, and a monoclonal antibody against hemagluttinin, α-HA. At right the images are merged. (F-J) indicates cells carrying V5 tagged proteins. The bar equals 2 μm.Aliens sifting through the remains of a lost human civilization might puzzle over the function of a ladle. But if found in a room associate
The Shape of Heredity
Susan M. Gasser | Jul 1, 2009 | 10+ min read
By Susan M. Gasser The Shape of Heredity Tracking the dance of DNA and structural proteins within the nucleus shows that placement makes the difference between gene activity and silence. What's true of the best architecture is also true of cellular structures: form follows function. We biologists often take this mantra to an extreme, searching for the function of a molecule or gene without much consideration of its structure, its phys

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