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tag dna synthesis culture

Keeping Tabs on Cultured Cells
Lissa Harris(lharris@the-scientist.com) | Mar 13, 2005 | 6 min read
THE CULTURE WITHIN:Photo courtesy of Drs. C.C. Uphoff and H.G. Drexler, DSMZ-German Collection of Microorganism and Cell Cultures, Braunschweig, GermanyHeLa cell line infected with Mycoplasma hyorhinis. Scanning electron micrograph of critical point-dried cell culture grown on cover slips. Note the loop- and rod-like mycoplasmas attached to the host cell membrane. Smaller web-like structures on the cell surface represent microvilli of the cell. Original magnification 3,000×.Mycoplasmas have
Premade cDNA Libraries
Sarah Goforth | Apr 15, 2001 | 10 min read
Suppliers of Whole-organism cDNA Libraries Suppliers of Tissue-specific cDNA Libraries The questions of gene function, interaction, and regulation are central to the science of molecular biology. Despite the myriad of new technologies, products, and techniques produced by the genomics revolution, some old standards remain just as useful as ever. One such technology is the cDNA (complementary DNA) library. The sheer number of companies offering premade and custom cDNA libraries and products th
Better Understanding Of Cell's Life Eases Culturing
Ricki Lewis | Nov 13, 1994 | 10+ min read
"Lots of companies come out with media or reagents for this or that, and make a big splash, but they're all basically derivatives of traditional products," says Hayden Coon, a former National Institutes of Health re-searcher who is the founder of Human Cell Therapies Inc. of Chebeague Island, Maine. Advanced Biotechnologies Inc. Columbia, MD American Qualex Antibodies La Mirada, CA American Type Culture Collection (ATCC) Rockv
Better Understanding Of Cell's Life Eases Culturing
Ricki Lewis | Nov 13, 1994 | 10+ min read
"Lots of companies come out with media or reagents for this or that, and make a big splash, but they're all basically derivatives of traditional products," says Hayden Coon, a former National Institutes of Health re-searcher who is the founder of Human Cell Therapies Inc. of Chebeague Island, Maine. Advanced Biotechnologies Inc. Columbia, MD American Qualex Antibodies La Mirada, CA American Type Culture Collection (ATCC) Rockv
Illustration showing a puzzle piece of DNA being removed
Large Scientific Collaborations Aim to Complete Human Genome
Brianna Chrisman and Jordan Eizenga | Sep 1, 2022 | 10+ min read
Thirty years out from the start of the Human Genome Project, researchers have finally finished sequencing the full 3 billion bases of a person’s genetic code. But even a complete reference genome has its shortcomings.
Out, Damned Mycoplasma!
Kelly Rae Chi | Dec 1, 2013 | 8 min read
Pointers for keeping your cell cultures free of mycoplasma contamination
DNA, RNA Probes Help Investigators Narrow The Search For Genes
Holly Ahern | Nov 26, 1995 | 10+ min read
For Genes Author: Holly Ahern For a geneticist, pinpointing a mutation in a human gene that might be responsible for an inherited disease can be likened to the proverbial search for a needle in a haystack. While mo-lecular biologists sift through the 3 billion base pairs in the human genome in search of disease-causing genes, other scientists choose to approach the problem from the perspective of the mouse, a model system for mammal research. Because mice and humans are members of the same gr
DNA Probes Yield Expanded Research And Clinical Uses
Ricki Lewis | Jan 9, 1994 | 10 min read
Author: Ricki Lewis Date:January 10, 1994, pp.17 The following vendors develop and/or market DNA probes for a variety of research and diagnostic purposes. For information about specific services, products, and prices, please contact these companies directly. Lofstrand Laboratories 7961 Cessna Ave. Gaithersburg, Md. 20879 (310) 330-0111 Fax: (301) 948-9214 Microbiological Associates Inc. Life Sciences Center 9900 Blackwell R
High Fidelity PCR: Enhancing the Accuracy of DNA Amplification
Shane Beck | Jan 4, 1998 | 10 min read
Date: January 5, 1998 Chart 1, Chart 2 n the beginning there was Taq. Actually, there were others before Taq. There were precursory polymerases, such as that from E. coli, that lost their enzymatic activities at elevated temperatures. This shortfall made thermal cycling a time-consuming chore, with the necessity of adding new polymerase after each cycle. Then came the thermostable polymerases such as Taq DNA polymerase, which was isolated from the thermophilic, aerobic bacterium Thermus aquat
Don't Clone Alone
Laura Defrancesco | Sep 14, 1997 | 10 min read
Date: September 15, 1997 cDNA Library Kit Table and Species Chart Pre-made cDNA libraries and kits abound in the market to help you probe the secrets of gene regulation while minimizing the drudgery of cDNA library construction. The ability to analyze a cell's genetic read-out-to determine which of the 100,000 possible genes are actually being expressed in a cell or tissue-is to know what makes a cell what it is. This is a central issue in molecular biology. For decades, cDNA libraries-colle

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