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tag gene repressors culture

Unsilencing a Gene
Tia Ghose | Dec 21, 2011 | 3 min read
Scientists have found a way to reactivate a gene in mice that is silenced in a neurodevelopmental disorder called Angelman syndrome.
Show Me The Money: Prokaryotic Expression Vectors And Purification Systems
Thomas F Unger | Sep 1, 1997 | 10+ min read
Face it, you're an agent! Scientists should really consider themselves professional promoters-promoters not too different from the type portrayed in a not-to-be-mentioned recent movie (it made enough money). Our clients are not jocks looking for some franchise to pick up a multi-year contract; our clients are genes that have biologically-athletic capabilities. Consider that genes are not just average run-of-the-mill sequences playing weekend pick-up games whenever they get enough friends togeth
Surpassing the Law of Averages
Jeffrey M. Perkel | Sep 1, 2009 | 7 min read
By Jeffrey M. Perkel Surpassing the Law of Averages How to expose the behaviors of genes, RNA, proteins, and metabolites in single cells. By necessity or convenience, almost everything we know about biochemistry and molecular biology derives from bulk behavior: From gene regulation to Michaelis-Menten kinetics, we understand biology in terms of what the “average” cell in a population does. But, as Jonathan Weissman of the University of Califo
PCR Based Cloning Kits: Something For Everybody
Laura Defrancesco | Apr 12, 1998 | 10+ min read
Date: April 13, 1998PCR Based Cloning Kits Table The End Table (PDF Format) PCR has found applications in almost every imaginable facet of molecular biology, and for many applications, looking at a band on a gel is not enough. Sequencing, expressing, mutating--all require cloning. And as it happens, cloning strategies that work for other types of DNA fragments don't work at all well, or require inordinate effort, with PCR fragments. For example, the most commonly used cloning strategy requires

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