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tag gas chromatography mass spectrometry cell molecular biology ecology

Mass Spectrometry Applications for Proteomics
Jeffrey Perkel | Aug 19, 2001 | 10+ min read
Click to view the PDF file: Proteomic Mass Spectrometry Equipment Courtesy of CiphergenCiphergen's SELDI process, a MALDI variant that includes a surface-based enrichment step Early in the twentieth century, scientists puzzled over the observation that certain elements that were otherwise physically indistinguishable from each other nevertheless exhibited different radioactive decay characteristics. These elements would ultimately come to be known as isotopes, but at the time this concept was
What Kind of Mass Spec User are You?
Jeffrey M. Perkel | Feb 1, 2007 | 5 min read
Whether studying proteins, nucleic acids, or small molecules, there's an ideal configuration for everyone.
Surpassing the Law of Averages
Jeffrey M. Perkel | Sep 1, 2009 | 7 min read
By Jeffrey M. Perkel Surpassing the Law of Averages How to expose the behaviors of genes, RNA, proteins, and metabolites in single cells. By necessity or convenience, almost everything we know about biochemistry and molecular biology derives from bulk behavior: From gene regulation to Michaelis-Menten kinetics, we understand biology in terms of what the “average” cell in a population does. But, as Jonathan Weissman of the University of Califo
Designing a More Accurate Protein Census
Douglas Steinberg | Apr 1, 2001 | 4 min read
Graphic: Leza BerardoneApplied Biosystems will soon begin testing new mass spectrometry machines designed to identify proteins in as many as 1,000 samples per hour. For the machines to work as planned, however, each sample must be prefractionated down to just a handful of proteins, according to Stephen A. Martin, director of the company's proteomics research center in Framingham, Mass. This example suggests how the slower protocols leading to mass spec are as important to the progress of proteom
Cracking the Complex
Jeffrey M. Perkel | Nov 1, 2015 | 8 min read
Using mass spec to study protein-protein interactions
New Technology Spurs on Proteomics
Jennifer Fisher Wilson | Apr 1, 2001 | 7 min read
Graphic: Leza BerardoneOne recent morning at the Applied Biosystems proteomics research center in Framingham, Mass., scientist Jason Marchese patiently used a pipettor to place tiny samples onto a 2-inch-by-2-inch plate. He was surrounded by technology as simple as 2-D gel electrophoresis apparatus and as cutting-edge as a high-throughput system that uses automated robotics for multidimensional liquid chromatography separation of proteins and an automated workstation that uses the latest in mass
Capillary Electrophoresis Gains Acceptance As Applications Increase
Howard Goldner | May 1, 1995 | 10 min read
Through the years, a variety of separation techniques have been used by researchers in their continuing challenge to accurately separate and identify analytes of interest from complex mixtures. Numerous procedures, physical as well as chemical, conducted in an assortment of vessels, are often used to achieve this goal. During the past 25 years, column techniques such as gas chromatography (GC) and high-performance liquid chromatography (HPLC) have led to successful separation and identification
2020 Top 10 Innovations
The Scientist | Dec 1, 2020 | 10+ min read
From a rapid molecular test for COVID-19 to tools that can characterize the antibodies produced in the plasma of patients recovering from the disease, this year’s winners reflect the research community’s shared focus in a challenging year.
Change of Expression
Steve Bunk | Jul 8, 2001 | 3 min read
For this article, Steve Bunk interviewed Rudolf Aebersold, cofounder, Institute for Systems Biology, Seattle. Data from the Web of Science (ISI, Philadelphia) show that Hot Papers are cited 50 to 100 times more often than the average paper of the same type and age. S.P. Gygi, Y. Rochon, B.R. Franza, R. Aebersold, "Correlation between protein and mRNA abundance in yeast," Molecular and Cellular Biology, 19:1720-30, March, 1999. (Cited in 114 papers) At virtually all levels of life sciences, fro
Mix Two Parts Quadrupole, One Part Ion Trap, and Stir
Jeffrey Perkel | Aug 18, 2002 | 3 min read
Photo: Courtesy of Applied Biosystems/MDS Sciex THE Q TRAP SYSTEM Last October, long-time mass spectroscopist Gerard Hopfgartner got a new toy. Applied Biosystems (AB) and its partner, MDS Sciex, sent Hopfgartner, professor of pharmaceutical analytical chemistry at the University of Geneva, a prototype instrument that combines the attributes of triple- quadrupole and ion trap mass spectrometers in a novel, hybrid configuration. Hopfgartner uses the instrument for both small-molecule and p

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