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tag agarose developmental biology

Beyond Film: Laboratory Imagers
Jorge Cortese | Apr 1, 2002 | 9 min read
Years ago, researchers had only one data-imaging option: autoradiography. These scientists tagged samples—whether nucleic acid, protein, cell, or tissue—with radioactive labels, and captured images on film. Safety concerns, convenience, and sensitivity, spurred the development of alternative techniques, and today, researchers can choose from a range of options, including fluorescence, chemifluorescence, and chemiluminescence, in addition to autoradiography. Fluorescence occurs when
Antibodies Making Their Way From The Clinic To The Research Lab
Holly Ahern | Sep 17, 1995 | 10 min read
if (n == null) The Scientist - The Immune Response The Scientist 9[18]:18, Sep. 18, 1995 Tools The Immune Response By Holly Ahern Imagine that you've just cloned a gene for a bacterial enzyme with unique structural properties and you want to find out more about it. What natural role does the enzyme play, you wonder, and do organisms other than bacteria produce it? To answer these questions, you could screen countless genomic libraries for clones bearing si
Assessing Differential Gene Expression
Barbara Cunningham | Nov 25, 2001 | 9 min read
As the complete human genome sequence emerges, research shifts from questions of genomics to those of proteomics--determining the function of individual gene products and mapping global gene expression patterns. Gene expression patterns change continually during the course of tissue development and differentiation. The expression of different gene products at any given time within a particular cell defines the cell's characteristics and helps determine how it will react to external stimuli. Alte

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