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tag quantitative pcr microbiology cell molecular biology ecology

Molecule of DNA forming inside the test tube equipment.3d rendering,conceptual image.
EvaGreen® Dye: The Swiss Army Knife of qPCR
Biotium | Mar 1, 2024 | 7 min read
A green fluorescent dye with a novel DNA binding mechanism improves signal-to-noise in different DNA amplification assays.
Quantitative PCR Update
Wendy Gloffke | Apr 20, 2003 | 9 min read
Courtesy of Applied Biosystems  THE TAQMAN PROCEDURE: Applied Biosystems' TaqMan procedure relies on the 5'-to-3' exonuclease activity of Taq polymerase. The TaqMan probe bears a fluoro-phore at the 5' end and a quencher on the 3' end, rendering the molecule non-fluorescent. During amplification, the probe binds to the template between the two PCR primers. When the polymerase encounters the probe, it starts chewing away at the end, releasing the fluorophore into solution, where it is free
bacteria and DNA molecules on a purple background.
Engineering the Microbiome: CRISPR Leads the Way
Mariella Bodemeier Loayza Careaga, PhD | Mar 15, 2024 | 10+ min read
Scientists have genetically modified isolated microbes for decades. Now, using CRISPR, they intend to target entire microbiomes.
Innovations Expand Lab Power, Uses Of PCR Technique
Ricki Lewis | Jul 25, 1993 | 8 min read
The gene amplification technique invented by genetics researcher Kary Mullis on a moonlit drive through the northern California hills a decade ago--the polymerase chain reaction (PCR)-- continues to revolutionize the life sciences. Uses in molecular biology research and in diagnostic tests are proliferating, and PCR is even bringing a new molecular approach to such fields as paleontology and epidemiology. The following companies are among those supplying PCR-related products for the resear
Artist’s rendering of a light purple Ebola virus looping around itself that’s surrounded by red blood cells
Ebola Lurking in Brain Fluid Kills Monkeys Weeks After Recovery
Dan Robitzski | Feb 9, 2022 | 4 min read
New research reveals where the virus was hiding and hints at how to truly purge it from the body.
Layered visual representation of multiomics
Integrate and Innovate with NGS and Multiomics
The Scientist and Illumina | May 4, 2023 | 6 min read
Researchers across disciplines combine layers of discovery obtained with accessible NGS-based multiomics approaches.
The Researchers Who Pivoted to COVID-19: One Year On
Shawna Williams | Jul 1, 2021 | 7 min read
The Scientist checks in on scientists who switched gears to combat the pandemic.
A scanning electron micrograph of a coculture of E. coli and Acinetobacter baylyi. Nanotubes can be seen extending from the E. coli.
What’s the Deal with Bacterial Nanotubes?
Sruthi S. Balakrishnan | Jun 1, 2021 | 10+ min read
Several labs have reported the formation of bacterial nanotubes under different, often contrasting conditions. What are these structures and why are they so hard to reproduce?
The Great Big Clean-Up
Kerry Grens | Sep 1, 2015 | 10+ min read
From tossing out cross-contaminated cell lines to flagging genomic misnomers, a push is on to tidy up biomedical research.
PCR Primed To Spur Chain Of Applications
Holly Ahern | Jun 25, 1995 | 10+ min read
What would you do if your research interests revolved around obtaining DNA from a bacterium preserved for millions of years in the gut of a bee stuck in amber, matching up a murderer to crime- scene blood half a century old, or cloning genes from a 1,000- year-old mummy? Most scientists would first consider PCR--the polymerase chain reaction--as a technique for approaching problems such as these. With PCR, minute quantities of nucleic acids can be amplified millions of times into sufficient qua

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