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tag quantitative pcr cell molecular biology neuroscience genetics genomics

Green and red fluorescent proteins in a zebrafish outline the animal’s vasculature in red and lymphatic system in green in a fluorescent image. Where the two overlap along the bottom of the animal is yellow.
Serendipity, Happenstance, and Luck: The Making of a Molecular Tool
Shelby Bradford, PhD | Dec 4, 2023 | 10+ min read
The common fluorescent marker GFP traveled a long road to take its popular place in molecular biology today.
Molecule of DNA forming inside the test tube equipment.3d rendering,conceptual image.
EvaGreen® Dye: The Swiss Army Knife of qPCR
Biotium | Mar 1, 2024 | 7 min read
A green fluorescent dye with a novel DNA binding mechanism improves signal-to-noise in different DNA amplification assays.
Different colored cartoon viruses entering holes in a cartoon of a human brain.
A Journey Into the Brain
Danielle Gerhard, PhD | Mar 22, 2024 | 10+ min read
With the help of directed evolution, scientists inch closer to developing viral vectors that can cross the human blood-brain barrier to deliver gene therapy.
Quantitative PCR Update
Wendy Gloffke | Apr 20, 2003 | 9 min read
Courtesy of Applied Biosystems  THE TAQMAN PROCEDURE: Applied Biosystems' TaqMan procedure relies on the 5'-to-3' exonuclease activity of Taq polymerase. The TaqMan probe bears a fluoro-phore at the 5' end and a quencher on the 3' end, rendering the molecule non-fluorescent. During amplification, the probe binds to the template between the two PCR primers. When the polymerase encounters the probe, it starts chewing away at the end, releasing the fluorophore into solution, where it is free
Bio-Rad 
Leveraging Molecular Technologies to Stay on Top of the Scientific Game
The Scientist Staff | Oct 26, 2022 | 5 min read
Joel Pearson discusses how emerging genomic technologies are shaping his cancer research.
Layered visual representation of multiomics
Integrate and Innovate with NGS and Multiomics
The Scientist and Illumina | May 4, 2023 | 6 min read
Researchers across disciplines combine layers of discovery obtained with accessible NGS-based multiomics approaches.
Researchers in George Church&rsquo;s lab modified wild type ADK proteins (left) in <em >E.coli</em>, furnishing them with an nonstandard amino acid (nsAA) meant to biocontain the resulting bacterial strain.
A Pioneer of The Multiplex Frontier
Rashmi Shivni, Drug Discovery News | May 20, 2023 | 10 min read
George Church is at it again, this time using multiplex gene editing to create virus-proof cells, improve organ transplant success, and protect elephants.
Chromatogram peaks of a DNA sequencing analysis.
Brush Up: What Is Bisulfite Sequencing and How Do Researchers Use It to Study DNA Methylation?
Deanna MacNeil, PhD | Sep 15, 2022 | 4 min read
Prior to DNA methylation sequencing, researchers treat their samples with sodium bisulfite to distinguish methylated cytosine from unmethylated cytosine.
Venus flytrap plants grow in the lab, trigger hairs at the ready.
How the Venus Flytrap Captures Its Prey
Rachael Moeller Gorman | Oct 16, 2023 | 4 min read
Scientists used CRISPR-Cas9 for the first time in a carnivorous plant to prove the role of two ion channels in closing the Venus flytrap’s trap.
A rendering of a human brain in blue on a dark background with blue and white lines surrounding the brain to represent the construction of new connections in the brain.
Defying Dogma: Decentralized Translation in Neurons
Danielle Gerhard, PhD | Sep 8, 2023 | 10+ min read
To understand how memories are formed and maintained, neuroscientists travel far beyond the cell body in search of answers.

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