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tag quantitative pcr cell molecular biology

A scientist loads a 96-well plate containing qPCR reactions into a thermal cycler for DNA amplification and detection.
Insights into qPCR: Protocol, Detection Methods, and Analysis
Tanuka Biswas, PhD | Nov 8, 2023 | 6 min read
Learn about quantitative PCR (qPCR), including its fundamentals, common applications, modes of detection, and key quantification methods.
Green and red fluorescent proteins in a zebrafish outline the animal’s vasculature in red and lymphatic system in green in a fluorescent image. Where the two overlap along the bottom of the animal is yellow.
Serendipity, Happenstance, and Luck: The Making of a Molecular Tool
Shelby Bradford, PhD | Dec 4, 2023 | 10+ min read
The common fluorescent marker GFP traveled a long road to take its popular place in molecular biology today.
Molecule of DNA forming inside the test tube equipment.3d rendering,conceptual image.
EvaGreen® Dye: The Swiss Army Knife of qPCR
Biotium | Mar 1, 2024 | 7 min read
A green fluorescent dye with a novel DNA binding mechanism improves signal-to-noise in different DNA amplification assays.
Quantitative PCR Update
Wendy Gloffke | Apr 20, 2003 | 9 min read
Courtesy of Applied Biosystems  THE TAQMAN PROCEDURE: Applied Biosystems' TaqMan procedure relies on the 5'-to-3' exonuclease activity of Taq polymerase. The TaqMan probe bears a fluoro-phore at the 5' end and a quencher on the 3' end, rendering the molecule non-fluorescent. During amplification, the probe binds to the template between the two PCR primers. When the polymerase encounters the probe, it starts chewing away at the end, releasing the fluorophore into solution, where it is free
Close up image of an open notebook with blank pages, a computer keyboard, and a pen.
What's Your Story? Competition Guidelines and Writing Tips
The Scientist | Nov 29, 2023 | 7 min read
Enter for a chance to have your story published on The Scientist’s website! 
Bio-Rad 
Leveraging Molecular Technologies to Stay on Top of the Scientific Game
The Scientist Staff | Oct 26, 2022 | 5 min read
Joel Pearson discusses how emerging genomic technologies are shaping his cancer research.
Venus flytrap plants grow in the lab, trigger hairs at the ready.
How the Venus Flytrap Captures Its Prey
Rachael Moeller Gorman | Oct 16, 2023 | 4 min read
Scientists used CRISPR-Cas9 for the first time in a carnivorous plant to prove the role of two ion channels in closing the Venus flytrap’s trap.
Quantitating Glycans for Biomarker Discovery
Amanda Keener | Aug 27, 2017 | 8 min read
Techniques for comparing the relative abundances of glycans and glycoproteins in clinical samples
Tag, You're It
Carina Storrs | Feb 1, 2014 | 7 min read
A guide to DNA-encoded libraries for drug discovery
Innovations Expand Lab Power, Uses Of PCR Technique
Ricki Lewis | Jul 25, 1993 | 8 min read
The gene amplification technique invented by genetics researcher Kary Mullis on a moonlit drive through the northern California hills a decade ago--the polymerase chain reaction (PCR)-- continues to revolutionize the life sciences. Uses in molecular biology research and in diagnostic tests are proliferating, and PCR is even bringing a new molecular approach to such fields as paleontology and epidemiology. The following companies are among those supplying PCR-related products for the resear

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