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tag quantitative pcr disease medicine cell molecular biology microbiology

Molecule of DNA forming inside the test tube equipment.3d rendering,conceptual image.
EvaGreen® Dye: The Swiss Army Knife of qPCR
Biotium | Mar 1, 2024 | 7 min read
A green fluorescent dye with a novel DNA binding mechanism improves signal-to-noise in different DNA amplification assays.
Quantitative PCR Update
Wendy Gloffke | Apr 20, 2003 | 9 min read
Courtesy of Applied Biosystems  THE TAQMAN PROCEDURE: Applied Biosystems' TaqMan procedure relies on the 5'-to-3' exonuclease activity of Taq polymerase. The TaqMan probe bears a fluoro-phore at the 5' end and a quencher on the 3' end, rendering the molecule non-fluorescent. During amplification, the probe binds to the template between the two PCR primers. When the polymerase encounters the probe, it starts chewing away at the end, releasing the fluorophore into solution, where it is free
bacteria and DNA molecules on a purple background.
Engineering the Microbiome: CRISPR Leads the Way
Mariella Bodemeier Loayza Careaga, PhD | Mar 15, 2024 | 10+ min read
Scientists have genetically modified isolated microbes for decades. Now, using CRISPR, they intend to target entire microbiomes.
Artist’s rendering of a light purple Ebola virus looping around itself that’s surrounded by red blood cells
Ebola Lurking in Brain Fluid Kills Monkeys Weeks After Recovery
Dan Robitzski | Feb 9, 2022 | 4 min read
New research reveals where the virus was hiding and hints at how to truly purge it from the body.
Digital PCR as a Universal Molecular Diagnostic Standard
Bio-Rad | Sep 30, 2020 | 6 min read
A reliable primary reference measurement procedure is the key to many forms of measurement across a diverse range of scientific research. The ability of digital PCR to quantify nucleic acids without the need for calibration will likely make it an essential tool for laboratories around the world to calibrate their measurements against. 
Combating COVID-19 with Cell-Free Expression
The Scientist | Aug 19, 2020 | 6 min read
Scientists rely on synthetic biology and cell-free expression systems for developing novel approaches to combat the pandemic.
New Molecular Tools Enable Researchers To Correlate Viruses, Diseases
Karen Young Kreeger | Feb 4, 1996 | 7 min read
Viruses, Diseases Author: Karen Young Kreeger Sidebar: Professional Resources for Viral Disease Researchers In the mid- to late 1980s, numerous correlations were discovered between viruses and various types of cancers. For example, Epstein-Barr virus was associated with nasopharyngeal carcinoma and B-cell lymphoma, hepatitis B virus with liver cancer, and human papillomavirus with cervical cancer. Now, a decade later, basic and clinical scientists are finding out that viruses may also play a r
Innovations Expand Lab Power, Uses Of PCR Technique
Ricki Lewis | Jul 25, 1993 | 8 min read
The gene amplification technique invented by genetics researcher Kary Mullis on a moonlit drive through the northern California hills a decade ago--the polymerase chain reaction (PCR)-- continues to revolutionize the life sciences. Uses in molecular biology research and in diagnostic tests are proliferating, and PCR is even bringing a new molecular approach to such fields as paleontology and epidemiology. The following companies are among those supplying PCR-related products for the resear
The AIDS Research Evaluators
Lynn Gambale | Jul 9, 1995 | 6 min read
Chairman: Arnold Levine, chairman, department of molecular biology, Princeton University Barry Bloom, Weinstock Professor and Howard Hughes Medical Institute (HHMI) investigator, department of microbiology and immunology, Albert Einstein College of Medicine, New York Rebecca Buckley, professor of pediatrics and immunology, Duke University Medical Center Charles Carpenter, chairman, Office of AIDS Research Advisory Committee; professor of medicine,Brown University School of Medicine Don
Do Microbes Trigger Alzheimer’s Disease?
Jill U. Adams | Sep 1, 2017 | 10 min read
The once fringe idea is gaining traction among the scientific community.

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