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tag ice caps cell molecular biology

DNA molecule.
Finding DNA Tags in AAV Stacks
Mariella Bodemeier Loayza Careaga, PhD | Mar 7, 2024 | 8 min read
Ten years ago, scientists put DNA barcodes in AAV vectors, creating an approach that simplified, expedited, and streamlined AAV screening. 
Peptide, Oligonucleotide Synthesis: Key In Molecular Studies
James Kling | Jun 8, 1997 | 9 min read
HIGH THROUGHPUT: PE Applied Biosystems’ ABI 394 DNA/RNA Synthesizer is controlled by a Macintosh computer. Polypeptides (proteins) and polynucleotides (DNA/RNA) are the two essentials of organic life. Just as detailed blueprints and clay bricks are essential to construction workers, shorter synthetic versions of these two molecular workhorses-peptides and oligonucleotides-are crucial to experimental biologists. Biologists put oligonucleotides to nearly as many uses as Mother Nature does
A New View of Translational Control
Charles Choi(cchoi@the-scientist.com) | Dec 4, 2005 | 7 min read
The bank note that Dominique Weil used to buy ice cream for her family at the beach this past summer may have traveled a long way.
Capturing Cancer Cells on the Move
Nicholette Zeliadt | Apr 1, 2014 | 9 min read
Three approaches for isolating and characterizing rare tumor cells circulating in the bloodstream
Cell-free Transcription and Translation
Gregory Smutzer | Jan 7, 2001 | 8 min read
In Vitro Translation Systems Researchers synthesize recombinant proteins in cell-free extracts to verify the identity of cloned genes, to study protein-protein, protein-nucleic acid, and protein-drug interactions, and to carry out mutagenesis studies. In vitro protein translation studies rely on the efficient and selective transcription of cloned genes in vitro, which is now not only possible, but also routine, thanks to the identification of bacteriophage (phage) DNA-dependent RNA polymerases a
Freeze Frame
Jeffrey M. Perkel | Feb 1, 2009 | 10 min read
How to troubleshoot sample preparation for cryo-electron microscopy, an up-and-coming structural biology technique.
Cell-Free And Happy: In Vitro Translation And Transcription/Translation Systems
Deborah Wilkinson | Jun 20, 1999 | 8 min read
Date: June 21, 1999Kits for In Vitro Translation and Transcription/Translation Systems Problem proteins--you know the ones. Some are rapidly degraded by endogenous proteins. Others are toxic to the very cells used to overexpress them. These types of problems can sometimes be avoided by using cell-free extracts for the in vitro expression of proteins. In vitro translation systems are also useful for other applications, including incorporating modified or unnatural amino acids for functional st
Diffusion in the Ranks
Michael Brush | Feb 4, 2001 | 8 min read
Suppliers of Dialysis Tubing Suppliers of Dialysis Devices (Not included in print edition) Dialysis effects the simple, gentle, and gradual separation of biological and other molecules from unwanted molecules in solution by selective diffusion through a semipermeable membrane. In practice, a sample of protein or nucleic acid that contains an unwanted small molecular weight compound, such as a buffer salt, is placed on one side of a semipermeable membrane. The dialysate, a solution of low ionic
Temples of Science
Paula Park | Jun 1, 2003 | 10+ min read
Image courtesy of Magnus Stark The Broad Center for the Biological Sciences, at the California Institute of Technology, designed by Pei Cobb Freed & Partners James Spudich, a Stanford University biochemist, likens the cell to a city. It incorporates roads and pathways, he says, and houses large structures, akin to buildings, such as the mitochondria and nucleus. But unlike the city, the cell can completely transform its own structure according to its needs. The right signals can convert t
Hit Parade
Amber Dance | Dec 1, 2012 | 9 min read
Cell-based assays are popular for high-throughput screens, where they strike a balance between ease of use and similarity to the human body that researchers aim to treat.

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