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Optimizing PCR: Proven Tips and Troubleshooting Tricks
Optimizing PCR: Proven Tips and Troubleshooting Tricks
Tanuka Biswas, PhD | Feb 23, 2024 | 10 min read
Scientists optimize PCR to ensure successful amplification and analysis.
Tag, You're It
Carina Storrs | Feb 1, 2014 | 7 min read
A guide to DNA-encoded libraries for drug discovery
Probing Questions
Michael Brush | May 1, 2000 | 7 min read
Nucleic Acid Labeling Kits Nucleic Acid Labeling Kits (continued) Nucleic Acid Labeling Kits (continued) Genisphere's 3DNA™ Expression Array Detection Kit reagents The ability to label nucleic acids with a variety of tagged molecules has led to astonishing advances in molecular biology. Labeled DNA, RNA, and oligonucleotide probes have made possible techniques like the historic Southern blot, the northern blot, and related dot/slot-blotting procedures, in addition to in situ hybridizat
Illustration showing a puzzle piece of DNA being removed
Large Scientific Collaborations Aim to Complete Human Genome
Brianna Chrisman and Jordan Eizenga | Sep 1, 2022 | 10+ min read
Thirty years out from the start of the Human Genome Project, researchers have finally finished sequencing the full 3 billion bases of a person’s genetic code. But even a complete reference genome has its shortcomings.
Monoclonal Antibodies Find Utility In Cell Biology
Ricki Lewis | Dec 11, 1994 | 10+ min read
But, just as antibodies are finding increasing utility in cell biology, a new Food and Drug Administration classification for those products with clinical utility may affect researchers' access to the important technology (see accompanying story). Monoclonal History MAbs were born in 1975, when Georges Kohler and Cesar Milstein at the Medical Research Council Laboratories in Cambridge, England, fused two types of cells to form a hy
Monoclonal Antibodies Find Utility In Cell Biology
Ricki Lewis | Dec 11, 1994 | 10+ min read
But, just as antibodies are finding increasing utility in cell biology, a new Food and Drug Administration classification for those products with clinical utility may affect researchers' access to the important technology (see accompanying story). Monoclonal History MAbs were born in 1975, when Georges Kohler and Cesar Milstein at the Medical Research Council Laboratories in Cambridge, England, fused two types of cells to form a hy
High Fidelity PCR: Enhancing the Accuracy of DNA Amplification
Shane Beck | Jan 4, 1998 | 10 min read
Date: January 5, 1998 Chart 1, Chart 2 n the beginning there was Taq. Actually, there were others before Taq. There were precursory polymerases, such as that from E. coli, that lost their enzymatic activities at elevated temperatures. This shortfall made thermal cycling a time-consuming chore, with the necessity of adding new polymerase after each cycle. Then came the thermostable polymerases such as Taq DNA polymerase, which was isolated from the thermophilic, aerobic bacterium Thermus aquat
DNA, RNA Probes Help Investigators Narrow The Search For Genes
Holly Ahern | Nov 26, 1995 | 10+ min read
For Genes Author: Holly Ahern For a geneticist, pinpointing a mutation in a human gene that might be responsible for an inherited disease can be likened to the proverbial search for a needle in a haystack. While mo-lecular biologists sift through the 3 billion base pairs in the human genome in search of disease-causing genes, other scientists choose to approach the problem from the perspective of the mouse, a model system for mammal research. Because mice and humans are members of the same gr
Array of Options
Jorge Cortese | May 28, 2000 | 10 min read
Instrumentation for Microarray Production and Analysis - Part 1 Instrumentation for Microarray Production and Analysis - Part 2 Nanogen's NanoChip™ Cartridge Today's molecular biology era can be defined by the dictum, "So many genes, so little time," and technologies for gathering genetic information are gaining speed. DNA microarrays are one of the most promising answers to that cry. DNA microarrays are glass microslides or nylon membranes containing DNA samples (genomic DNA, cDNA,
Innovative Alternatives To PCR Technology Are Proliferating
Ricki Lewis | Jan 20, 1991 | 8 min read
Since the earliest days of the biotechnology endeavor, scientists have sought a practical way of co-opting the complex biological machinery that regulates DNA. From the moment they identified the intriguing enzymes that choreograph the dance of DNA replication, biotechnologists began developing ways to commander them; the swivelases and gyrases, forms of unwinding proteins that untwist the molecule; the nucleases, which snip it; the strands; the ligases, which tie these strands together; and ev

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