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tag pcr immunology genetics genomics culture

A Test Bed for Budding Technologies
Aileen Constans | Jul 4, 2004 | 6 min read
DELETION BY DESIGN:Courtesy of Guci GiaeverThe deletion cassette module used to delete each yeast gene contains two 74-basepair tags upstream and downstream (UPTAG and DNTAG) of the KanMX gene, which confers resistance to the drug geneticin. UPTAG and DNTAG contain 18 basepairs of genomic sequence to flank the yeast's open reading frame, and U1 and U2, or D1 and D2 PCR primers for amplifying a unique 20-basepair TAG region-the so-called molecular barcode. A second round of PCR adds 45 base-pairs
Top 10 Innovations 2021
2021 Top 10 Innovations
The Scientist | Dec 1, 2021 | 10+ min read
The COVID-19 pandemic is still with us. Biomedical innovation has rallied to address that pressing concern while continuing to tackle broader research challenges.
PCR Primed To Spur Chain Of Applications
Holly Ahern | Jun 25, 1995 | 10+ min read
What would you do if your research interests revolved around obtaining DNA from a bacterium preserved for millions of years in the gut of a bee stuck in amber, matching up a murderer to crime- scene blood half a century old, or cloning genes from a 1,000- year-old mummy? Most scientists would first consider PCR--the polymerase chain reaction--as a technique for approaching problems such as these. With PCR, minute quantities of nucleic acids can be amplified millions of times into sufficient qua
Making Things Grow: Insect Cells, Stem Cells, and Primary Cell Lines All Pose Challenges for Cell Culturists
Laura Defrancesco | Jun 21, 1998 | 5 min read
Date: June 22, 1998 Insect Cell Culture Media, Suppliers of Primary Cell Culture Media Advantages for Protein Expression Studies Since the mid-1950s cultures of insects--cockroaches, fruit flies, and leafhoppers, to name a few--have been the object of quiet study by physiologists and cell biologists. But along came genetic engineering and suddenly insect cultures have been put in the spotlight since they provide advantages over both bacterial and mammalian systems for recombinant protein prod
 coronavirus covid-19 sars-cov-2 diagnostic test real-time pcr antibodies cdc
How SARS-CoV-2 Tests Work and What’s Next in COVID-19 Diagnostics
Bianca Nogrady | Mar 3, 2020 | 6 min read
Current methods to detect infections of the novel coronavirus rely on identifying particular genetic sequences, but new assays are being developed to meet the growing demand for rapid answers.
Top 10 Innovations 2013
The Scientist | Dec 1, 2013 | 10+ min read
The Scientist’s annual competition uncovered a bonanza of interesting technologies that made their way onto the market and into labs this year.
The Leprosy Watcher
Tom Hollon | Jun 23, 2002 | 8 min read
Volume 16 | Issue 13 | 15 | Jun. 24, 2002 Previous | Next The Leprosy Watcher Armed with recent genomics data, Bill Levis ponders leprosy's immunological fork in the road--and awaits a government decision regarding his own career | By Tom Hollon Graphic: Marlene J. Viola Patients come to him by referral, dreading what they may hear after being poked and palpated and scrutinized by one puzzled
Research Briefs
Maria Anderson | Oct 19, 2003 | 4 min read
Research Briefs New Genes: The Ears Have 'Em; The Worker, The Soldier, The Candlestick Maker; For Genomes Without Borders, Biobanks Unite New genes: The ears have 'em While scouring a new cDNA library, researchers in the Netherlands discovered 80 novel expressed-sequence tags, including 25 preferentially expressed in human fetal cochlea.1 The researchers from the University Medical Center in Nijmegen (UMCN) found that 155 ESTs map to loci for nonsyndromic deafness, which is not associated
Week in Review: October 21–25
Tracy Vence | Oct 25, 2013 | 4 min read
PubMed launches Commons; measuring HIV’s latent reservoir; immune-related pathway variation in genome, microbiome; rapamycin and flu vaccines; grasshopper mice resistant to pain
2020 Top 10 Innovations
The Scientist | Dec 1, 2020 | 10+ min read
From a rapid molecular test for COVID-19 to tools that can characterize the antibodies produced in the plasma of patients recovering from the disease, this year’s winners reflect the research community’s shared focus in a challenging year.

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