Golden Retriever

Seminiferous vessels stained for cytokeratin 18 without (upper) and with (lower) antigen retrieval Chemically fixing tissue sections may lock in a good result for the world to see, but it can complicate subsequent immunohistochemical staining by masking relevant antigens. A process known as antigen retrieval can restore the antigenicity of proteins altered by fixation and improve immunohistochemical detection. It can also reduce background staining, which makes it an important step in developing

By | June 12, 2000


Seminiferous vessels stained for cytokeratin 18 without (upper) and with (lower) antigen retrieval
Chemically fixing tissue sections may lock in a good result for the world to see, but it can complicate subsequent immunohistochemical staining by masking relevant antigens. A process known as antigen retrieval can restore the antigenicity of proteins altered by fixation and improve immunohistochemical detection. It can also reduce background staining, which makes it an important step in developing a standardized protocol. Until recently, antigen was typically retrieved manually using a microwave oven and special solutions. While this method generally produces good results, variables such as solution temperature, microwave wattage, and the size and color of the container holding the solution can affect the quality, reproducibility, and reliability of results. With the introduction of the GenoMx1000 (patent pending), the first fully automated antigen retrieval system, BioGenex Laboratories Inc. of San Ramon, Calif., standardizes and streamlines this important immunohistochemistry pretreatment procedure.

Combining BioGenex's patented microwave technology and antigen retrieval solutions, the GenoMx 1000 performs three individual functions: dewaxing (to remove paraffin), simultaneous dewaxing and antigen retrieval, and dewaxing followed by H&E staining. It can process up to 300 slides at one time and performs multiple antigen retrieval protocols (for up to 100 slides) simultaneously. According to Philipp Novales-Li, director of scientific affairs at BioGenex, the GenoMx 1000 optimizes tissue processing for further applications such as automated staining by the GenoMx 6000: "When you automate antigen retrieval and staining, you control all the potential parameters that could lead to erroneous results or low reproducibility." Novales-Li says that the system represents one stage in BioGenex's plan to create five modules for the eventual full automation of immunohistochemistry methods: sectioning, cutting, antigen retrieval, staining, and image analysis.

The GenoMx 1000 will be beneficial for clinical diagnostics, genomics research, and drug discovery laboratories. It is of primary interest for clinical pathology labs, where standardized antigen retrieval will help streamline in situ testing.

--Aileen Constans (aconstans@the-scientist.com)

For More Information

BioGenex Laboratories Inc.
(800) 421-4149
www.biogenex.com
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