CILIA FOR ASYMMETRY:
Courtesy of Joseph R. Marszalek and Lawrence S.B. Goldstein
Cilia from a mouse embryo found on the embryonic node. Researchers have shown that embryos without the genes encoding motor subunits for such cilia develop defects in left-right body asymmetry determination.
Most biologists are familiar with motile cilia, the finger-like appendages that allow unicellular organisms to swim, and the specialized cells that move fluids and clear away debris in our kidneys and lungs. Few are aware, though, that nearly every cell in the human body also possesses a single immotile or "primary" cilium. The functions of primary cilia are quite obscure, and until recently they were considered to be vestigial.
Ciliary biology has undergone a quiet revolution. Defects in primary cilia are now proposed to contribute to such diverse disorders as diabetes, obesity, and schizophrenia. At the same time, ciliary biologists are gaining a better understanding of disorders caused by defects in motile cilia, such as chronic respiratory disease and abnormal organ development. "There is quite a broad range of human disorder that is going to be caused by ciliary dysfunction," predicts Nicholas Katsanis of Johns Hopkins University, who studies Bardet-Biedl syndrome, a genetic disorder of ciliary origin.
This revolution largely resulted from the groundbreaking discovery of intraflagellar transport, or IFT, the process by which cells form cilia and flagella. IFT's discovery led directly to the finding that defects in primary cilia of kidney cells cause a common genetic disorder, polycystic kidney disease (PKD), which sparked renewed interest in these once overlooked organelles.
Researchers are working to identify the estimated 300 to 500 proteins that make up all cilia, as well as those involved in IFT. New studies of PKD and other disorders are showing that defects in primary and motile cilia can have profound effects on cell division and human development. While the exact functions of primary cilia are still unknown, growing evidence suggests that these organelles act as cellular antennae, receiving chemical or mechanical signals critical to controlling growth, differentiation, and proliferation.
UNCONTROLLED CELL DIVISION
In the roughly 1 in 1,000 people affected by PKD, multiple fluid-filled cysts develop in their kidneys due to uncontrolled cell proliferation. The end result is often renal failure. Much research in mice and humans has been devoted to understanding this devastating disorder. Yet, the ground-breaking results came from seemingly unrelated work on the unicellular, flagellated green algae,
In the early 1990's, researchers in Joel Rosenbaum's laboratory at Yale University* observed tiny particles moving up and down the length of
Rosenbaum's group soon cloned 18 or so genes involved in IFT. They searched Genbank for homologs. "One in particular stood out," he says. "When this gene was absent in mouse, it had all the characteristics of autosomal dominant PKD." These mice likely lacked the ability to assemble some essential cilium. But while primary cilia were known to exist on kidney cells, "they'd been relegated to the dustbin of being unimportant for half a century," he says. He and collaborators examined kidney tubules in the affected mice using scanning electron microscopy; they found that primary cilia were indeed either stunted or entirely absent.1
Courtesy William J. Snell and Jummin Pan
A fluorescence micrograph of the biflagellated green alga, Chlamydomonas reinhardtii, shows its ~12 μm-long flagella. Chlamydomonas use these microtubule filled organelles not only for motility, but also for cilia-generated signaling during sensory transduction.
They were puzzled at first because most human cases of PKD are caused by defects in two proteins seemingly unrelated to cilia. Polycystin 1, a receptor-like protein, and polycystin 2, a calcium channel, form a complex in the cell membrane. Greg Pazour and his group at the University of Massachusetts Medical School put the pieces together when they showed that these two proteins normally reside on primary cilia of kidney cells.2 If the cilia are defective, these proteins cannot properly localize and are prevented from carrying out their functions.
But these functions are still unknown. Jing Zhou and her group at Harvard Medical School have shown that bending the primary kidney cilium causes an influx of calcium through polycystin 2, suggesting that the cilium acts as a mechanoreceptor.3 This hypothesis is controversial, because a link has yet to be established between calcium influx in the cilium and control of cell division, says Pazour. "Polycystin 2 mutants show no calcium influx when the cilium is bent, but whether that is why you get PKD is still unknown," he says.
It has long been thought that primary cilia might be involved in control of cell division. Studies done in the 1970s showed that ciliary microtubules are formed using the same centrioles that organize the mitotic spindle, and primary cilia are well known to disappear and reappear during different cell cycle phases.
Donald Ingber, a coauthor on the Zhou study and also at Harvard, suggests a mechanism that might link ciliary bending to cell proliferation. Ingber studies cellular response to mechanical forces, particularly during development when cells are experiencing many changes in their external environment. "Distortion of the cytoskeleton activates signaling cascades [that] regulate apoptosis, growth, and differentiation," he says. And while it is a "bigger jump to say that forces on a cilium might affect these processes, the cilium
© 2003 Current Opinion
A primary cilium stained green (antibody to acetylated tubulin) in a cultured human retinal pigmented epithelial cell. The actin cytoskeleton appears in red (phalloidin), and the nuclei in blue (DAPI). Scale bar = 5 μm. (From G.J. Pazour, G.B. Witman, Curr Opin Cell Biol, 15:105–10, 2003.)
Other genetic disorders show a connection between ciliary dysfunction and development. Primary ciliary dyskinesia, or PCD, is frequently caused by mutations in the dynein arms of microtubules in motile cilia. Patients with PCD have the defects one might expect given the known functions of motile cilia in human tissues, according to Brian Hackett of Washington University, St. Louis, who studies lung development. Symptoms of PCD include recurrent respiratory infections due to inadequate lung clearance, and fertility problems caused by defective sperm and poorly functioning oviducts.
But these mutations have less obvious effects as well. Patients with a form of PCD known as Kartagener syndrome have a condition called
Other genetic disorders imply a role for primary cilia in development as well. Katsanis and his group study Bardet-Biedl syndrome (BBS), a complex genetic disorder recently found to be ciliary in origin. Patients with BBS show multiple, seemingly unrelated defects, including increased tendencies toward obesity and diabetes, polydactyly (extra fingers or toes), retinal degeneration, kidney malformation, and
"A year and a half ago, I knew nothing about cilia," says Katsanis. Then his group cloned the
But the implications go beyond this very rare disease. Katsanis emphasizes that the study of BBS has identified a specific cellular defect whose role can now be investigated in more common conditions. "We are studying BBS because it is multisystemic, hitting essentially every system in the human body," says Katsanis. "The same principles are going to be applicable to some experimentally intractable systems, such as diabetes, obesity, and schizophrenia."
The connection between primary cilia and disorders such as obesity is only a matter of speculation for now. Katsanis suggests that a predisposition to gain weight might be caused by ciliary defects in some specific organ. He points out that this organ could be the brain. Obesity might be a "neurodevelopment issue, caused by changes in the wiring of the hypothalamus," he says. Such changes might prevent proper operation of the feedback loop leading to satiation, for example.
The idea that primary cilia might affect brain development is supported by the observation that nearly all brain neurons possess a primary cilium on their cell bodies. He and others have detected changes in brain development in mice with ciliary dysfunction. The prevalence of mental disorders in patients with BBS also lends support. And recent studies show that primary cilia on some neurons carry serotonin and somatostatin receptors, potential pathways for developmental signals.
A CELLULAR ANTENNA
Clearly, ciliary dysfunction can have profound effects on development in complex organisms. Researchers are now looking at cilia with known functions to get clues as to how primary cilia might be involved in development. They are focusing on the fact that many cilia are involved in sensory functions.
In vertebrates, "Cilia are critical for sensory perception; we see, smell, and hear with cilia," says William Snell, who studies the sensory role of the flagellum in
Beds of sensory cilia in the nose play a key role in olfaction. Katsanis and his colleagues recently showed that patients with BBS have impaired ability to smell, a previously unknown symptom. He and Randall Reed, who studies olfaction at Johns Hopkins, plan to exploit the olfactory system as a model for how defects in BBS-linked genes prevent proper ciliary function. They also plan to study the roles of these genes in olfaction.
Both primary and motile cilia are well known to play a sensory role in simpler organisms such as
Polycystin 1 is a very large membrane protein containing more than 4,000 amino acid residues. Besides eleven transmembrane spans, much of the protein (50%–75%) forms a large extracellular domain. Barr hypothesizes that polycystin 1 and LOV-1 are receptors that receive signals from the outside environment. But, "what activates them may be different," she says. "The mating signal could be chemical, or mechanical, or both." And clearly the consequences of signal reception are different in the two systems as well. She plans to take advantage of the well-established knowledge of
There is no shortage of candidate genes. Cilia are extremely complex organelles, composed of hundreds of proteins. Recently, several groups have compared the genomes of ciliated organisms, such as humans and
From the proteomics side, Greg Pazour's lab has identified at least 300 proteins in the
Megan M. Stephan