Time-lapsed confocla microscopic images of T-cells undergoing apoptosis. The green fluroescence (a cyanine dye, which indicates a mitochondrial membrane potential) marks healthy cells, while the red fluorescence is derived from PhipPhiLux cleavage in apoptotic cells.
- they work both in solution and in cells, in common buffered solutions found in research labs and in bodily fluids encountered in clinical settings. They can be used with quartz as well as plastic cuvettes and in high throughput formats (microtiter plate, for example)
- they have the ability to penetrate membranes, enabling the study of proteolytic activity in live, intact cells
- intracellular protease activity can be visualized using a standard fluorescence microscope, as well as by multi-parameter flow cytometry
- the substrates contain amino acid sequences from both sides of cleavage sites, providing a degree of specificity not found with substrates made with only amino terminal information
- the substrates mimic active site loop conformation of proteases and thus provide a three-dimensional target for proteases.
For more information, visit the Oncoimmunin web site at www.access.digex.net/~oncoim/