2D gels are not enough

A series of narrow-pH-range overloaded 2D gels, processed using sensitive silver-staining, yields enough spots that the gels could, theoretically, be surveying virtually all of the proteins of budding yeast. But in the August 15 Proceedings of the National Academy of Sciences, Gygi et al. report that proteomics proponents may have been fooling themselves (Proc Natl Acad Sci USA 2000, 97:9390-9395). Gygi et al. select a random region of one such gel and analyze all the visible spots. The genes co

By | August 17, 2000

A series of narrow-pH-range overloaded 2D gels, processed using sensitive silver-staining, yields enough spots that the gels could, theoretically, be surveying virtually all of the proteins of budding yeast. But in the August 15 Proceedings of the National Academy of Sciences, Gygi et al. report that proteomics proponents may have been fooling themselves (Proc Natl Acad Sci USA 2000, 97:9390-9395). Gygi et al. select a random region of one such gel and analyze all the visible spots. The genes corresponding to the identified proteins have a codon bias characteristic of high or medium abundance proteins. Many of these proteins are present in multiple spots, whereas many lower abundance proteins that would be expected to appear in the selected region (based on predicted molecular weight and pI) are not detected. This suggests that more sensitive chromatographic processes may be needed for complete proteomics analyses.

Popular Now

  1. Antarctica Is Turning Green
  2. How to Tell a Person’s “Brain Age”
  3. Male Fish Borrows Egg to Clone Itself
  4. Life Science Funding Cuts Leaked
    The Nutshell Life Science Funding Cuts Leaked

    According to a document posted online less than a day before the release of the official 2018 budget proposal, the National Institutes of Health could face even deeper cuts than previously suggested by the Trump administration.

AAAS