in vitro

Cloning works because even highly differentiated somatic nuclei can de-differentiate and reacquire their ability to form all the cells of the body. The de-differentiation process is driven by egg cytoplasm, and in the 29 September Science Kikyo et al. report that the chromatin remodeling protein ISW1 is probably one component of the de-differentiation machinery (Science 2000, 289:2360-2362). Kikyo et al. identify a number of proteins that are released from permeabilized frog somatic nuclei only

By | October 5, 2000

Cloning works because even highly differentiated somatic nuclei can de-differentiate and reacquire their ability to form all the cells of the body. The de-differentiation process is driven by egg cytoplasm, and in the 29 September Science Kikyo et al. report that the chromatin remodeling protein ISW1 is probably one component of the de-differentiation machinery (Science 2000, 289:2360-2362). Kikyo et al. identify a number of proteins that are released from permeabilized frog somatic nuclei only in the presence of frog egg cytoplasm plus ATP. Fractionation of the egg cytoplasm yields ISW1, a member of the SWI2/SNF2 superfamily of chromatin remodeling proteins. Other chromatin remodeling proteins are not active in the assay, but unidentified proteins are needed in addition to ISW1. Further discoveries in this field may allow for cloning, or the derivation of stem cells, without the need to use egg cells.

Popular Now

  1. Antarctica Is Turning Green
  2. How to Tell a Person’s “Brain Age”
  3. Male Fish Borrows Egg to Clone Itself
  4. Life Science Funding Cuts Leaked
    The Nutshell Life Science Funding Cuts Leaked

    According to a document posted online less than a day before the release of the official 2018 budget proposal, the National Institutes of Health could face even deeper cuts than previously suggested by the Trump administration.

AAAS