Advertisement
Ingenuity
Ingenuity

in trans

Experiments with irradiated mice show that damage to DNA in the paternal genome is hazardous for the maternally derived genome.

By | February 16, 2001

Irradiation of pre-meiotic stage germ cells was shown to cause germline mutation of mouse minisatellite sequences. In the February 13 Proceedings of the National Academy of Science, Niwa and Kominami report on the effect of irradiation on paternal and maternal minisatellite alleles (Proc Natl Acad Sci USA 2001, 98:1705-1710). They examined length change mutation of the mouse hypervariable minisatellite locus Ms6hm. Male mice were irradiated at the testicular level with 6 gray (Gy) of γ-ray irradiation and crossed with non-irradiated females. The mutant frequency of the paternal allele was 22% for males irradiated at the spermatozoa stage, compared with the 8.4% spontaneous background in non-irradiated parents. Hence, irradiation of post-meiotic germ cells induces minisatellite mutation in F1 mice. In addition, the mutant frequency at the maternally derived allele was also increased in F1 offspring (20% compared with 9.8% in controls) from irradiated fathers. These results imply that radiation-induced genomic instability causes untargeted mutation of paternal alleles in cis and maternal alleles in trans, and caution about the hazard of paternal DNA damage to the maternally derived genome upon radiation exposure.

Advertisement

Follow The Scientist

icon-facebook icon-linkedin icon-twitter icon-vimeo icon-youtube
Advertisement
Ingenuity
Ingenuity

Stay Connected with The Scientist

  • icon-facebook The Scientist Magazine
  • icon-facebook The Scientist Careers
  • icon-facebook Neuroscience Research Techniques
  • icon-facebook Genetic Research Techniques
  • icon-facebook Cell Culture Techniques
  • icon-facebook Microbiology and Immunology
  • icon-facebook Cancer Research and Technology
  • icon-facebook Stem Cell and Regenerative Science
Advertisement
The Scientist
The Scientist
Advertisement
The Scientist
The Scientist
Life Technologies