Support for histone code?
Four papers released online today detail some of the work from David Allis? group and others that?s detailed in our linkurl:recent article;http://www.the-scientist.com/2006/5/1/34/1/ on chromatin remodeling.
Four papers released online today detail some of the work from David Allis? group and others that?s detailed in our linkurl:recent article;http://www.the-scientist.com/2006/5/1/34/1/ on chromatin remodeling. In two __Nature__ papers released today, Allis, a Rockefeller chromatin researcher, along with postdocs Joanna Wysocka and Tomek Swigut and a team led by structural biologist Dinshaw Patel, from Memorial Sloan Kettering, report on BPTF, the largest subunit of the nucleosome remodeling factor (NURF). It contains a so-called PHD finger which they?ve linkurl:now shown;http://www.nature.com/nature/journal/vaop/ncurrent/abs/nature04815.html preferentially binds histone 3 trimethylated at lysine 4. They found the protein in pull-down assays and it appears to maintain activity at developmentally critical __HOX__ genes. This work is notable for adding support to the linkurl:controversial histone code;http://www.the-scientist.com/article/display/23393/ hypothesis.
The linkurl:structural work;http://www.nature.com/nature/journal/vaop/ncurrent/abs/nature04802.html reveals an alpha helix between the PHD finger and a bromodomain. Bromodomain motifs are well known to recognize acetylated lysines, so that unstructured alpha helix might be acting as a spacer between the two recognition motifs. The authors write ?The extent to which paired PHD finger-bromodomain in BPTF recognizes combinatorialy methyl and acetyl marks in one or multiple tails, in keeping with the histone code hypothesis remains to be determined.?
I saw Allis give a very engaging talk at Thomas Jefferson University last Thursday, and he was hinting that the ?multiple tails? option was looking pretty good.
But just in case the relationship between PHD fingers and gene activity sounded straightforward enough, the two other __Nature__ papers released at the same time (and presumably being published in the same print issue) complicate issues, revealing that the PHD domain of tumor suppressor ING2 also recognizes trimethylated H3K4. This is puzzling because ING2 is closely associated with the mSin3a-HDAC1 histone deacetylase, a complex that represses gene transcription. Or Gozani at Stanford, Tatiana Kutateladze at University of Colorado Health Science Center and their respective collaborating groups published two papers describing the linkurl:function;http://www.nature.com/nature/journal/vaop/ncurrent/abs/nature04835.html and linkurl:structure;http://www.nature.com/nature/journal/vaop/ncurrent/abs/nature04814.html of ING2 and its ability to block gene transcription after DNA damage. This is the first protein to link H3K4 methylation to transcriptional repression, and it does it through the same recognition motif (the PHD finger) that Allis? papers describe. They write, ?Together, our findings highlight the notion that the recognition of chromatin modifications by effector proteins rather than specific modification __per se__, determines biological function, and, as such, greatly expands the diversity of signaling at chromatin.?
Allis told me he thought it was fascinating that the same mark (H3K4 trimethylated) might signal both expression and repression. Some researchers argue that if the same signal gives different, even opposite readouts , then there can?t be a predictive system. But the context of other signals around H3K4 methylation may play a role in this and other situations.
July 30, 2006
It is not so surprising that the same modification can recruit activators or repressors. The "histone code" concept has to be framed in a dynamic context. You may need to recruit activators to translate the histone modification (tri-methyl H3K4) into transcriptional activation, and into epigenetic inheritance of such activation or its potential; but at the time of reprogramming the gene, you need to recognize the active chromatin to deactivate transcription by recruiting a repressor.