Advertisement
Logos Biosystems
Logos Biosystems

Time and Temperature

By Richard P. Grant Time and Temperature Joseph Takahashi (An artistic representation of the SCN in black on a colorful background) The paper E. D. Buhr et al., “Temperature as a universal resetting cue for mammalian circadian oscillators,” Science, 330:379-85, 2010. Free F1000 Evaluation The finding The body’s circadian rhythms are regulated by a “master clock” in the brain, whose tempo is set by light-dark cycles, while t

By | February 1, 2011

Time and Temperature

Joseph Takahashi (An artistic representation of the SCN in black on a colorful background)

The paper

E. D. Buhr et al., “Temperature as a universal resetting cue for mammalian circadian oscillators,” Science, 330:379-85, 2010.
Free F1000 Evaluation

The finding

The body’s circadian rhythms are regulated by a “master clock” in the brain, whose tempo is set by light-dark cycles, while the peripheral clocks that control liver metabolism or pituitary hormone release can be reset by a number of stimuli, including temperature. Joseph Takahashi and colleagues at UT Southwestern Medical Center showed that the suprachiasmatic nucleus (SCN) itself was unaffected by temperature, but that it most likely used body temperature to synchronize the peripheral clocks.

The reporter

Takahashi’s group fused the green luciferase reporter to the mouse clock gene Per2, to watch both the SCN and peripheral clocks glow as they cycled. Changing the temperature of lung and pituitary tissues changed the rhythm of Per2 expression, but didn’t affect the SCN clock. “Light’s a great way to reset the SCN,” says F1000 Member Stacey Harmer, who evaluated the paper, but it’s the “daily cycles in body temperature that help the peripheral oscillators keep time with the brain clock.”

The thermometer

Takahashi’s lab also inhibited heat shock factor 1 (HSF1) in lung and pituitary cultures. Without HSF1, the tissues were unable to respond to increased temperature, implicating heat shock proteins as the thermometer that changes gene expression in peripheral clocks.

The resistance

How the SCN resists temperature changes is Takahashi’s new problem, he says. He aims to determine the cellular and synaptic mechanisms that might allow the SCN to respond to light, but not to temperature.

F1000 evaluators: P. Fuller (UC Davis) & C. Saper (Harvard Med School) R. Mistlberger (Simon Fraser Univ) S. Harmer (UC Davis)

Advertisement

Popular Now

  1. Antibody Alternatives
    Features Antibody Alternatives

    Nucleic acid aptamers and protein scaffolds could change the way researchers study biological processes and treat disease.

  2. The Mycobiome
    Features The Mycobiome

    The largely overlooked resident fungal community plays a critical role in human health and disease.

  3. Circadian Clock and Aging
    Daily News Circadian Clock and Aging

    Whether a critical circadian clock gene is deleted before or after birth impacts the observed aging-related effects in mice.

  4. Biologist Resigns Amid Sexual Misconduct Probe
Advertisement
INTEGRA
INTEGRA
Advertisement
Life Technologies