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Come Inside

By Richard P. Grant Come Inside Lesley McKeane, MRC Visual Aids (virus) The paper D.L. Mallery et al., “Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21),” PNAS, 107:19985-90, 2010. Free F1000 Evaluation The finding Antibodies work by activating the complement cascade, preventing invading microorganisms from entering cells, or binding to a pathogen and marking it for destruction by immune cells. Now,

By | March 1, 2011

Come Inside

Lesley McKeane, MRC Visual Aids (virus)

The paper

D.L. Mallery et al., “Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21),” PNAS, 107:19985-90, 2010. Free F1000 Evaluation

The finding

Antibodies work by activating the complement cascade, preventing invading microorganisms from entering cells, or binding to a pathogen and marking it for destruction by immune cells. Now, Leo James and colleagues at the MRC-LMB in Cambridge have discovered a fourth method that works inside cells, providing a “last line of defense” against infection.

The ambush

James’ lab observed that antibodies can remain bound to adenovirus as it infects cells. The result sparked their curiosity, because they had previously shown that the intracellular protein TRIM21 binds to the Fc region of antibodies with higher affinity than any other antibody receptor in the body.



Animated Immunity

Watch antibodies neutralize viral invaders in this animation from the lab of Leo James
Credit: Lesley McKeane, MRC Visual Aids


The neutralization

They found that TRIM21 adds ubiquitin to the adenovirus-antibody complex, targeting it for destruction by the proteasome. The finding “adds conceptually to the immune response,” says F1000 Member David Alpers.

The patents

TRIM21 only works if the virus doesn’t shed the antibody as it enters the cell. This means it should be effective against all nonenveloped viruses, such as rotaviruses and noroviruses, which cause diarrhea and gastroenteritis. James’ lab is in the process of cataloging which infections retain antibodies, and looking for other TRIM21-like proteins. They are also trying to develop novel antiviral therapies, having already applied for patents covering the use of synthetic TRIM21 to treat infections.

F1000 evaluators: L. Walter (German Primate Center) D. Alpers (WUSM)N. Vigneron & B. Van den Eynde (Ludwig Inst for Cancer Res) G. Versteeg & A. Garcia-Sastre (Mount Sinai Sch of Med)D. Koch & N. Sawtell (Cincinnati Children’s Hosp Med Cen)

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Comments

Avatar of: Dov Henis

Dov Henis

Posts: 97

March 3, 2011

Epigenetics: A Long Overdue Pre-Primer\n[Comment posted 2011-03-01]\nhttp://www.the-scientist.com/2011/3/1/32/1/\n\n\nScience Should Adjust Its Vision, Comprehension And Concepts\n\nFrom "Think Again: Education" , comment "Science In A Technology-Tradeunion Culture?"\nhttp://www.foreignpolicy.com/articles/2011/02/22/think_again_education\n\nGenes And Genomes Are Both Organisms\n\nGenomes Are RNA-Evolved Template ORGANISMS \n\nEpiDNAtics Is Not Epigenetics\n\n\nFrom "Dispel Some Figments Of 2010 Science Imagination"\nhttp://pulse.yahoo.com/_2SF3CJJM5OU6T27OC4MFQSDYEU/blog/articles/245540\n\nThe "heritable or enduring changes" are epiDNAtics, not epigenetics. Alternative splicing is not epigenetics, even if/when not involving alteration of the DNA sequence. Earth life is an RNA world.\n\nIt's the RNAs that evolve proteins. AND IT'S THE RNAs THAT HAVE EVOLVED AND PRODUCE AND EMPLOY THE RNA and (stabler) DNA template genome organisms for carrying out life processes, i.e. for enhancing Earth's biosphere by proliferating RNAs, for augmenting and constraining as long as possible some energy by augmenting its, RNA's, self-propagation, constraining temporarily some of the total energy of the universe, all of which is nevertheless destined to fuel the ongoing cosmic expansion. \n\nIT HAS ALWAYS BEEN AND IT STILL IS AN RNA EARTH LIFE.\n\nScience should adjust its vision, comprehension and concepts. \n\n\nDov Henis \n(comments from 22nd century)\nSeed of Human-Chimp Genomes Diversity\nhttp://pulse.yahoo.com/_2SF3CJJM5OU6T27OC4MFQSDYEU/blog/articles/53079\n03.2010 Updated Life Manifest\nhttp://www.the-scientist.com/community/posts/list/54.page#5065

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