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Taking Aim at Melanoma

By Keith T. Flaherty Taking Aim at Melanoma Understanding oncogenesis at the molecular level offers the prospect of tailoring treatments much more precisely for patients with advanced cases of this deadliest of skin cancers. Pep Karsten / fstop / Corbis They’re lawyers and receptionists, philanthropists and film editors. Some are retired, some just starting families. What they have in common is metastatic melanoma, a cancer that will likely claim their

By | April 1, 2011

Taking Aim at Melanoma

Understanding oncogenesis at the molecular level offers the prospect of tailoring treatments much more precisely for patients with advanced cases of this deadliest of skin cancers.

Pep Karsten / fstop / Corbis

They’re lawyers and receptionists, philanthropists and film editors. Some are retired, some just starting families. What they have in common is metastatic melanoma, a cancer that will likely claim their lives in a matter of months. Standard treatments—including removal of tumors and chemotherapy—have failed to halt their advancing disease. But these patients have not given up. All have volunteered to take part in clinical trials designed to test novel therapies that could help rein in their tumor growth and possibly buy them some time. And thanks to two promising new approaches, these patients—and others like them—have reason to be hopeful.

The new treatments are more carefully aimed than the massive but indiscriminate hammer blow dealt by standard chemotherapeutics, which have always disappointed in the treatment of melanoma. One approach takes advantage of drugs that have been designed specifically to take down the mutant protein that is inappropriately activated in more than half of all patients with melanoma. Another is a form of immunotherapy that activates T cells that can recognize and attack the tumors. Both have shown an ability to improve the lives of patients with metastatic melanoma, and both are likely to gain FDA approval this year. The results offer hope that someday we may be able to overcome this disease, for which no effective treatments currently exist.

Identifying a culprit gene

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1 These mutations inappropriately activate the kinase, which then turns on a second kinase called MEK. This activational cascade—part of the well-studied MAP kinase signaling pathway—ultimately turns on a suite of genes that mediates cell division. (See “2,3

4

Attacking BRAF

BRAF is a protein of 766 amino acids. The most common mutation found in melanoma changes the valine (V) at position 600 to a glutamic acid (E). This alteration—dubbed V600E in shorthand—accounts for 90 percent of the mutations in tumor samples analyzed. The next most common mutation, present in a handful of melanomas, changes that same valine to a lysine (V600K). A smattering of other mutation also occur in and around that amino acid hot spot.1

Although targeted BRAF inhibitors have shown promise for malignant melanoma, coming up with more effective combination therapies tops the agenda.

All these mutations function to lock BRAF in its active state. When the protein is inactive, a segment called the P-loop covers the site where ATP binds. When BRAF is activated, the P-loop swings out, exposing this site and allowing the kinase to phosphorylate its target protein, MEK.4 Structural studies, particularly of the V600E form of BRAF, show that mutations repel the P-loop, keeping it from shutting down the kinase activity. (See illustration above.)

The first targeted BRAF inhibitor was designed specifically to recognize and shut down the V600E form of the protein. Developed by scientists at a small biotech company called Plexxikon, the compound—PLX4032—binds to BRAF in its active conformation and prevents it from interacting with its target protein MEK.4 In blocking the activation of MEK, PLX4032 arrests progression into the G1 phase of the cell cycle, halting proliferation. And it also triggers apoptosis—in vitro, in animal models, and in the tumors of patients enrolled in Phase I/II clinical trials. 5

Next to bat

GSK2118436 is a drug developed by the pharmaceutical company GlaxoSmithKline (GSK). Like PLX4032, the GSK compound is a highly selective inhibitor of mutant forms of BRAF. Both bind to the same region of the protein and help hold it in its inactive conformation. But the GSK compound is even more potent than PLX4032: it cuts BRAF’s activity in half at concentrations less than 1 nM. Clinical trials for this potent inhibitor have accepted patients who have any form of the V600 mutant BRAF proteins. Most of those enrolled have the V600E form of mutant BRAF, but a few have the V600K or V600G (valine to glycine) mutation.

Although GSK2118436 has been administered to fewer patients than PLX4032, both drugs have shown similar efficacies in terms of tumor shrinkage. At the maximal dosages, about 90 percent of patients with BRAF mutations show some degree of tumor regression; 60 to 70 percent of patients had tumors that shrank by one-third or more.6 The trials have not gone on long enough to assess either drug’s effect on overall survival, but compared to treatment with dacarbazine, PLX4032 appears to triple the length of time patients have before the disease starts to worsen—from two months with dacarbazine to six months with the new drug.

The GSK compound had the added benefit of actually reducing the size of some of the small brain metastases seen in a subgroup of the patients enrolled in that trial.5 This response was particularly welcome because half of all patients with malignant melanoma will develop brain tumors, which respond particularly poorly to treatment. Once melanoma spreads to the brain, survival can be a matter of weeks, particularly for those with large, symptomatic metastases. So GSK2118436’s ability to shrink brain tumors is being actively pursued.

The trials have been an emotional roller coaster for the patients receiving the drugs—and for the researchers and medical personnel developing and administering them. [See this three-part series, “7 Other tumors accumulate mutations that activate different signaling pathways which mediate cell proliferation or survival, including that of another oncogenic kinase called phosphoinositide 3-kinase (PI 3-kinase).8

Getting a handle on how tumors can circumvent BRAF inhibitors should give us a better idea of how to attack melanomas that become resistant to these promising new agents. The knowledge could also point the way toward drugs that could be effectively combined with BRAF-targeted inhibitors as a frontline form of therapy for metastatic melanoma to prevent the emergence of these resistance mechanisms. BRAF inhibitors could be given along with targeted inhibitors of other oncogenic kinases, including PI 3-kinase. Another approach would be to combine targeted BRAF inhibitors with an entirely different type of treatment, such as immunotherapy.9

The long haul

Even if targeted molecular therapies like PLX4032 or the GSK compound were 100 percent effective, only half of all patients with melanoma have tumors with BRAF mutations. For the other half, we need to come up with some viable alternatives. Some of the patients have other mutations that might serve the same function as BRAF. For those patients, similar treatment strategies are being developed. But for any patient with melanoma, immunotherapy provides an option that does not appear to depend on specific mutations in the tumor.

10 CTLA4 inhibits T-cell activation and helps to promote immunologic tolerance, so blocking this protein should stimulate an immune response and help prime T cells to attack tumors bearing antigenic target proteins.

The results of the first large phase III trial for ipilimumab look promising. In this trial, investigators compared patients who received either a combination of the gp100 vaccine plus ipilimumab to those who received either of the treatments on its own. In this case, the vaccine-only patients served as a “control arm” of the study, since gp100 has not been found to be effective in treating melanoma when administered as a single agent. The results confirmed what had been seen in smaller, nonrandomized studies in which everyone received the drug: about 10 percent of the patients taking ipilimumab—either with or without the peptide vaccine—experienced tumor shrinkage of one-third or more.11 As yet, we do not know why these patients responded to the drug, while others did not. One goal for the future would be to identify what makes these responders unique so that we can target this therapy to the patient population most likely to benefit.

Even though 90 percent of the patients showed no shrinkage, it does not necessarily mean that the drug did them no good. For some of these patients, ipilimumab (again, taken alone or in conjunction with the peptide vaccine) has halted disease progression: their tumors have remained the same size for years—and they are still alive to tell about it. Such a response is unusual for patients with malignant melanoma, a cancer that generally progresses quite rapidly. And among the 10 percent of patients who initially responded to the drug, most continue to benefit. In the randomized trial it was clear that a larger percentage of patients lived for two years after receiving ipilimumab, and from earlier trials some who have been responding for 10 years and counting.

Another large phase III trial—comparing ipilimumab plus dacarbazine to dacarbazine alone—has been going on for several years and we hope the results of that study will corroborate the life-prolonging effects we’ve already seen. But even without that trial, many of us believe that the FDA will look favorably on the results reported thus far, because no other drug tested to date has ever enhanced survival in a phase III trial for malignant melanoma.

More one-two punches

Although targeted BRAF inhibitors have shown promise, none of us is satisfied with what single-agent therapies have accomplished thus far. So for malignant melanoma, coming up with more effective combination therapies tops the agenda. The low-hanging fruit would involve using drugs already developed for targeting other kinases or other pathways in which aberrant activity contributes to the development of melanoma, such as members of the PI 3-kinase pathway.

At the same time, oncologists are migrating toward mixing different types of therapies, such as targeted BRAF inhibitors plus immunotherapy. Our group at Massachusetts General Hospital has produced data supporting the idea that combining those approaches would likely benefit our patients. We have found that melanoma cells exposed to BRAF inhibitors become more densely decorated with surface antigens such as gp100. In the absence of treatment, these cancer cells somehow suppress expression of their melanocyte-specific antigens, which presumably helps them to escape immune surveillance. And BRAF inhibitors seem to reverse this suppression. Working with melanoma biopsies taken before and after therapy with BRAF inhibitors, we have found that treated tumor cells display more melanocyte- specific antigens and are more easily recognized by T cells harvested from the patient.9

Of course, convincing pharmaceutical companies to make their drugs available for such combination trials can in itself be challenging. First, the specificity of each treatment could mean that any combination would further fractionate the population of patients for whom the treatment would work. For example, more than half of patients with melanoma have a mutation in BRAF. And a smaller percentage of those will have a BRAF mutation combined with a companion mutation in another kinase for which an inhibitor is available or can be developed. Drug companies can be loath to move forward with therapies for which the market promises to be small.

Pharmaceutical companies also shy away from coadministering drugs because of the fear that a drug combination could produce a unique toxicity—something that would not be an issue with either drug alone. Such a reaction could kill a compound that’s still in development—even if that drug would be perfectly safe and perhaps effective when taken as a single agent.

Cancer is a complex disease, and developing effective treatments is more complex still. For the benefit of patients, my colleagues and I contend that the whole drug development industry needs to recognize this complexity and incentives need to be provided to companies that are willing to take on the challenge of formulating new therapies—and combinations of therapies—to tackle this disease. The science is out there to support these approaches. Now, we as physicians need to show as much determination as our patients in our ongoing search for the drugs—or combinations of drugs—that will allow people with malignant melanoma to return home and go on living their lives.

Andrzej Tokarski / ISTOCKPHOTO.COM

A DIET OF PILLS

There comes a point in any trial when investigators agree to pause and take stock of the situation. In Phase I trials, for example, we typically stop to determine whether we’re seeing any side effects that would warrant discontinuing the trial—or whether we’re seeing any effects that would suggest we move forward and expand the number of patients receiving the drug. In the case of PLX4032, developed at a small California biotech company called Plexxikon, we ran into a different problem: we hit a plateau in terms of bioavailability. In other words, we could increase the dosage of drug the patients were receiving, but we couldn’t get their blood concentrations high enough.

On the one hand, we could have opted to push forward, persuading our patients to swallow even more of the poorly absorbed pills. But there are drawbacks to that approach. First, overloading people’s digestive systems with drugs can produce local irritation, resulting in diarrhea and the like. Although these conditions are not life-threatening, they can make it difficult for patients to tolerate the therapy—not a good outcome for any new drug. And even if the megadoses don’t cause intestinal distress, a high pill count can cause problems down the road. Patients in Phase I trials are a highly motivated cohort, willing to serve as guinea pigs on drugs that have never before been tested in humans. But what if patients in later trials are not as willing to do whatever it takes? If your drug only works at the highest dose, but your patients are not willing to swallow more than 30 pills a day to achieve that dose, then the trial will fail to show efficacy, and a potentially useful drug could be permanently shelved.

Instead, we voted to pause—to wait as long as it would take to derive a better formulation. If that formulation could not be found, we would consider walking away, trying to find another agent that could achieve the concentrations that would be needed to really take down the mutant BRAF. Fortunately, Plexxikon and Roche (the company’s new partner in developing PLX4032) were willing and able to rework the drug into a form that boosted blood concentrations higher. Even then, we saw no significant side effects, so we increased the dosage—to the point that people were taking 14 capsules, twice a day. That was a steady diet of pills.

Since then, the drug has been made even more concentrated, so fewer, smaller capsules will yield the same effect. It’s important to remember that we are working with people here—people we wish to help. So we have to respect their limits. In doing so, we not only benefit the patients in our trial, but all patients who will ultimately benefit from receiving an effective treatment in the future.

Editor's Note (4th April): Just prior to the publication of this article, the FDA approved ipilimumab for use in advanced melanoma patients. It was shown to lengthen the life spans of 20-30% of advanced melanoma patients participating in a clinical trial of the drug. Some patients lived several years after their initial diagnosis.

F1000 Member References:

1. H. Davies et al.,“Mutations of the BRAF gene in human cancers,” Nature, 417:949-54, 2002. Free F1000 Evaluation
2. K.T. Flaherty et al.,“Phase I/II, pharmacokinetic and pharmacodynamic trial of BAY 43-9006 alone in patients with metastatic melanoma,” J Clin Oncol, 23(16s): abstract 3037, 2005.
3. T. Eisen et al., “Sorafenib in advanced melanoma: a Phase II randomised discontinuation trial analysis,” Br J Cancer, 95:581-86, 2006.
4. G. Bollag et al., “Clinical efficacy of a RAF inhibitor needs broad target blockade in BRAF-mutant melanoma,” Nature, 467: 596-99, 2010. Free F1000 Evaluation
5. R. A. Kefford et al., “Phase I/II study of GSK2118436, a selective inhibitor of oncogenic mutant BRAF kinase, in patients with metastatic melanoma and other solid tumors,” J Clin Oncol, 28(15s): abstract 8503, 2010.
6. K. T. Flaherty et al., “Inhibition of mutated, activated BRAF in metastatic melanoma,” N Engl J Med, 363:809-19, 2010. Free F1000 Evaluation
7. C.M. Johannessen et al., “COT drives resistance to RAF inhibition through MAP kinase pathway reactivation,” Nature, 468:968-72, 2010. Free F1000 Evaluation
8. R. Nazarian et al., “Melanomas acquire resistance to B-RAF(V600E) inhibition by RTK or N-RAS upregulation,” Nature, 468:973-77, 2010.
9. A. Boni et al., “Selective BRAFV600E inhibition enhances T-cell recognition of melanoma without affecting lymphocyte function,” Cancer Res, 70:5213-19, 2010. Free F1000 Evaluation
10. J.S. Weber et al., “Phase I/II study of ipilimumab for patients with metastatic melanoma,” J Clin Oncol, 26:5950-56, 2008.
11. F.S. Hodi et al., Improved survival with ipilimumab in patients with metastatic melanoma, N Engl J Med, 363:711-23, 2010. Free F1000 Evaluation


This article is adapted from a review in F1000 Medicine Reports, DOI:10.3410/M3-8 (open access at http://bit.ly/hpJlK9). For citation purposes, please refer to that version.

Comments

Avatar of: Mike Waldrep

Mike Waldrep

Posts: 155

April 4, 2011

Interesting! I hope that everyone's Sunday was great and I hope that they have a great week. I also hope that they had a great April Fool's Day! That goes for last year and all the other years that I've missed.
Avatar of: john fryer

john fryer

Posts: 11

April 5, 2011

Just got one friend with this complaint. Not treated yet but will be soon.\n\nHe takes lots of sun tan cream and does not overly get in the sun.\n\nIs there anything harmful in the sun tan cream?\n\nMy own idea which works so far is to take no sun tan cream but use nature and caution to avoid over exposure.\n\nIt is difficult to imagine that what has been shining down on us for generations can suddenly cause epidemic skin cancer.\n\nThe other big suspect is radioactive material and the loss possibly of 600 000 plutonium rods from Fukushima and the effect on future health in 10 years or so may be illuminating.
Avatar of: Jonas Moses

Jonas Moses

Posts: 26

April 6, 2011

With all due respect, even Dr. Peter Duesberg no longer subscribes to the oncogene theory. Seeking to understand or treat cancer on the basis of oncogenetics and molecular models alone is barking up the wrong tree - trying to treat a disease that is not likely genetic in origin or nature. Cancer is many things - a change in microenvironment, due to prolonged exposure to toxic chemicals/radiation, toxic lifestyle or toxic medications; a response to chronic inflammation; a "wound that will not heal"; signatory of a deficient/damaged or suppressed immune system,...et al., yet, there is no good evidence that cancer is cellular misadventure due to genetic predisposition or catastrophic mutation. Just as tumor angiogenesis was debunked (Maniotis, et al.; see reference list, below), yet continues to surface, oncogenetics is failed science.\n\nSome suggested reading, for further illumination and discussion (decidedly, only a partial list):\n\n1) Maniotis A., Folberg R., Hess A., Seftor E., Gardner L., Pe?er J., Trent J., Meltzer P., Hendrix M. Vascular channel formation by human uveal melanoma cells in vivo and in vitro: Vasculogenic mimicry. Amer. J. Path. Vol. I55, No 3, pps. 739-752, September, 1999. \n\n3) Robert Folberg, Mary Hendrix, and Andrew Maniotis. Vasculogenic Mimicry and Tumor Angiogenesis. American Journal of Pathology, Vol 156, No.2, 2000. \n\n4) Michael A. Warso, Andrew Maniotis, Xue Chen, Dibyen Majumdar, Niu K. Patel, Anne Shilkaitis, tapas K. Das Gupta, Robert Folberg. Prognostic Significance of Vasculogenic mimicry patterns in primary cutaneous melanoma. Clin Cancer Res. Mar;7(3):473-7, 2001. \n\n5) Folberg R, Chen X, Boldt HC, Pe?er J, Brown CK, Woolson RF, Maniotis AJ: Microcirculation patterns other than loops and networks in choroidal and ciliary body melanomas. Ophthalmology, 98:996-1001, 2001. \n\n6) Andrew Maniotis, Xue Chen, Christopher Garcia, Phillip J. DeChristopher, Ding Wu, Jacob Pe?er, Robert Folberg. Control of Melanoma Morphogenesis Endothelial Survival, and Perfusion By Extracellular Matrix. Lab Investigation. Vol. 82 No. 8 p.1083-1092, 2002. \n\n7) A. Mueller, A. Maniotis, W. Freeman, D. Bartch, U.Schaller, G.Bergeron-Lynn, L. Cheng, Taskintuna, X. Chen, J. Kan-Mitchell, R. Folberg. An orthotopic model for human uveal melanoma in SCID mice. Microvasc Res Vol. 44, 2002. \n\n8) Chen X, Maniotis AJ, Majumdar D, Pe'er J, Folberg R: Uveal melanoma cell staining for CD34 and the assessment of tumor vascularity. Invest Ophthalmol Vis Sci. Aug; 43(8): 2533-9, 2002.\n\n9) Seftor EA, Meltzer PS, Kirschmann DA, Pe'er J, Maniotis AJ, Trent JM, Folberg R, Hendrix MJ. Molecular determinants of human uveal melanoma invasion and metastasis. Clin Exp Metastasis, 19(3) 233-46, 2002. \n\n10) Chen X, Ai Z, Rasmussen M, Bajcsy P, Auvil L, Welge M, Leach L, Vangveeravong S, Maniotis AJ, Folberg R. Three-dimensional reconstruction of extravascular matrix patterns and blood vessels in human uveal melanoma tissue: techniques and preliminary findings. Investigative Ophthalmology and Visual Science. 44:2834-2840, 2003. \n\n11) Richard Gordon, John Karavitis, Jonas Moses, Klara Valyi-Nagy, and Andrew Maniotis. Differentiation waves versus positional information of morphogen gradients: which is cause and effect? Information Processing in Cells and Tissues, Morphomechanics of the Embryo and Genome, Lausanne , Switzerland , 2003. \n\n12) Robert Folberg, Jacob Pe?er, Andrew J. Maniotis. Extravascular Matrix Patterns in Uveal Melanoma: Histogenesis, Structure, and Molecular Regulation. In: Uveal Melanoma: A model for Exploring Fundamental Cancer Biology. Swets & Zeitlinger, Publishers, 2004. \n\n13) Folberg R, Maniotis A. Vasculogenic mimicry. Acta Pathologica Microbiologica et Immunologica Scandinavica, 112:508-25, 2004. \n\n14) Maniotis AJ, Valyi-Nagy K, Karavitis J, Moses J, Boddipali V, Wang Y, Nuñez R, Setty S, Arbieva Z, Bissell MJ, and Folberg R: Chromatin organization measured by Alu I restriction enzyme changes with malignancy and is regulated by the extracellular matrix and the cytoskeleton. Am J Pathol 166: No. 4 April 2005. \n\n15) Lin AY, Maniotis AJ, Valyi-Nagy K, Majumdar D, Setty S, Kadkol S, Leach L, Pe'er J, Folberg R.Distinguishing fibrovascular septa from vasculogenic mimicry patterns. Arch Pathol Lab Med. Jul;129(7):884-92, 2005. \n\n16) Kadkol SS, Lin AY, Barak V, Kalickman I, Leach L, Valyi-Nagy K, Majumdar D, Setty S, Maniotis AJ, Folberg R, Pe'er J. Osteopontin expression and serum levels in metastatic uveal melanoma: a pilot study. Invest Ophthalmol Vis Sci. Mar;47(3):802-6, 2006. \n\n17) Folberg R, Arbieva Z, Moses J, Hayee A, Sandal T, Kadkol S, Lin AY, Valyi-Nagy K, Setty S, Leach L, Chevez-Barrios P, Larsen P, Majumdar D, Pe'er J, Maniotis AJ. Tumor cell plasticity in uveal melanoma: microenvironment directed dampening of the invasive and metastatic genotype and phenotype accompanies the generation of vasculogenic mimicry patterns. Am J Pathol. Oct;169(4):1376-89, 2006. \n\n18) Tone Sandal, Klara Valyi-Nagy, Robert Folberg, Mina Bissell, Virginia Spensor, Andrew Maniotis. Epigenetic reversion of breast carcinoma phenotype and DNA sequestration. American Journal Of Pathology, Vol. 170(5):1739-49. May, 2007. \n\n19) Klara Valyi-Nagy, Robert Folberg, Tibor Valyi-Nagy, Andrew J. Maniotis. Susceptibility of Herpes simplex Virus Type I and II, The Role of Tumor Invasiveness, The Extracellular Matrix, and Chromatin Sequestration. In press, May, Experimental Eye Research, Vol. 84, 9991-10,000, 2007. \n\n20) Lin AY, Ai Z, Lee SC, Bajcsy P, Pe'er J, Leach L, Maniotis AJ, Folberg R. Comparing vasculogenic mimicry with endothelial cell-lined vessels: techniques for 3D reconstruction and quantitative analysis of tissue components from archival paraffin blocks. Appl Immunohistochem Mol Morphol. Mar;15(1):113-9, 2007. \n\n21) Folberg R, Leach L, Valyi-Nagy K, Lin AY, Apushkin MA, Ai Z, Barak V, Majumdar D, Pe'er J, Maniotis AJ. Modeling the behavior of uveal melanoma in the liver. Invest Ophthalmol Vis Sci. 2007 Jul;48(7):2967-74. \n\n22) Barak V, Frenkel S, Kalickman I, Maniotis AJ, Folberg R, Pe'er J. Serum markers to detect metastatic uveal melanoma. Anticancer Res. Jul-Aug;27(4A):1897-900, 2007. \n\n23) Barak V., Frenkel S., Valyi-Nagy K., Leach L., Apushkin MA., Lin A., Kalickman I., Baumann N., Pe?er J., Maniotis A., Folberg R. Using the direct-injection model of early uveal melanoma hepatic metastasis to identify TPS as a clinically useful serum biomarker. Invest Ophthalmol Vis Sci. Oct;48(10):4399-402, 2007. \n\n24) Papadopulos-Eleopulos E, Page BA, Causer D, Turner VF, Papadimitriou JM, Maniotis A. Cancer and epigenetic reversion the fundamental role of redox. Am J Pathol. Nov;171(5):1726-7, 2007. \n\n25) Grace Guzman, Scott J. Cotler, Amy Y. Lin, Robert Folberg, Andrew J. Maniotis. Expression of vasculogenic mimicry correlates with earlier hepatocellular carcinoma recurrence after orthotopic liver transplant. Archives Lab. Medicine, Vol. 131, p 56-61, 2007. \n\n\n
Avatar of: LINDA TU

LINDA TU

Posts: 1

April 30, 2011

I found this article to be potentially useful as an educational aid in explaining the machanisms of cancer therapy to students who have an interest in cancer research and who may not have a medical background.

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