The Right Sort

Isolating specific cell types from a mass of plant or animal tissue is laborious and tricky. 

By | August 1, 2011

JPG | PDF George Retseck" > Infographic: The Right Sort
View full size JPG | PDF George Retseck

INFOGRAPHIC BY GEORGE RETSECK

Isolating specific cell types from a mass of plant or animal tissue is laborious and tricky. To study epigenetic changes and genes that are expressed differently in different cell lineages—such as cancer cells versus normal cells, or the two types of epidermal cells in Arabidopsis roots—typically requires laser capture microdissection (LCM) or fluorescence-activated cell sorting (FACS). LCM uses a laser and a microscope to literally flip individual cells out of a tissue into a container. It’s like playing tiddlywinks, says Elizabeth Dennis at CSIRO in Canberra, Australia, but you have to flip out a thousand individual cells for each experiment. “It’s a real pain,” she says. Like FACS, it also requires expensive equipment. Roger Deal and Steven Henikoff from the Fred Hutchinson Cancer Research Center have developed a cheap and easy method, dubbed “isolation of nuclei tagged in specific cell types” (INTACT).

Read the full story.

Add a Comment

Avatar of: You

You

Processing...
Processing...

Sign In with your LabX Media Group Passport to leave a comment

Not a member? Register Now!

LabX Media Group Passport Logo

Popular Now

  1. Major German Universities Cancel Elsevier Contracts
  2. Grass Routes
    Features Grass Routes

    Researchers are discovering a suite of new locations and functions of endocannabinoid receptors that play roles in sickness and in health.

  3. Studies Retracted After UCLA Investigation
  4. Trump Nominates Sam Clovis to Lead USDA Research
AAAS