Advertisement

Stockpiling Histones

Histones stored on lipid droplets in fly embryos provide a backup supply when newly synthesized ones are lacking.

By | February 1, 2013

EDITOR'S CHOICE IN MOLECULAR BIOLOGY

HISTONE STORES: Wild-type Drosophila embryos contain an abundance of H2B histones (top layer, left), while engineered embryos (right) do not.© COURTESY OF ZHIHUAN LI

The paper
Z. Li et al., “Lipid droplets control the maternal histone supply of Drosophila embryos,” Curr Biol, 22: 2104-13, 2012.

The finding
Excess free-floating histones are toxic to cells. Yet researchers have observed massive stores of maternally supplied histones, bound to lipid droplets, in Drosophila embryonic cells. To understand the function of these fat-associated histones, Michael Welte of the University of Rochester and his colleagues disrupted the synthesis of new histones in fly embryos and found support for the idea that lipid droplets help the embryo safely store the excess pre-made histones for times of need.

The partner
By purifying the droplets and isolating the associated proteins, Welte’s group identified a new protein, Jabba, which anchors histones to lipid droplets. When they engineered embryos that lacked Jabba, they found that lipid droplets were devoid of histones. Surprisingly, the embryos developed normally, illustrating that new histones made by the embryos were sufficient to keep them alive.

The function
However, when the researchers both prevented histone expression and wiped out the histones on lipid droplets, the embryos died. “We interpret that the histone store on lipid droplets is sort of a backup mechanism,” says Welte. “Usually, the embryo can probably get away with making its own histones, but if you stress [the histone supply] a little bit, then the histones on lipid droplets become crucial.”

The conservation
Akash Gunjan at Florida State University who was not involved in the study says that the rainy-day storage function of lipid droplets makes sense for organisms whose swift development doesn’t allow a lot of time for translation. “It’s a good idea for them to have a ready-made store of histones that they can quickly make use of for the rapid embryonic cell cycles,” he says.
 

Add a Comment

Avatar of: You

You

Processing...
Processing...

Sign In with your LabX Media Group Passport to leave a comment

Not a member? Register Now!

LabX Media Group Passport Logo

Follow The Scientist

icon-facebook icon-linkedin icon-twitter icon-vimeo icon-youtube
Advertisement
RayBiotech
RayBiotech

Stay Connected with The Scientist

  • icon-facebook The Scientist Magazine
  • icon-facebook The Scientist Careers
  • icon-facebook Neuroscience Research Techniques
  • icon-facebook Genetic Research Techniques
  • icon-facebook Cell Culture Techniques
  • icon-facebook Microbiology and Immunology
  • icon-facebook Cancer Research and Technology
  • icon-facebook Stem Cell and Regenerative Science
Advertisement
Hamamatsu
Hamamatsu
Advertisement
The Scientist
The Scientist
Life Technologies