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Stem Cells: Safe Haven For TB

Tuberculosis bacteria find shelter from drugs and the body’s defenses in bone marrow stem cells.

By | February 5, 2013

WIKIMEDIA, NIAIDMycobacterium tuberculosis (TB) hides out in stem cells deep within bone tissue, where it avoids detection from the immune system and drugs, according to a study published last week (January 30) in Science Translational Medicine.

“This is a very exciting story,” said Horacio Frydman, a microbiologist at Boston University who was not involved in the research. In 2006, Frydman and his Princeton University postdoc advisor, Nobel Laureate Eric Wieschaus, reported the first case of bacteria living in a stem cell niche, after observing Wolbachia invade the stem cells of the Drosophila melanogaster germline. "Hitching a ride in cells that are self-renewing is a great strategy for reinfecting tissues in the host”, continued Frydman, “and we always assumed that other bacteria should also be doing the same.”

Ninety percent of TB infections are cleared by the body’s immune defenses or by taking anti-TB medication. In the remaining 10 percent of cases, however, TB persists as a dormant, non-replicating infection. Though latent TB infections do not cause symptoms, they can reactivate after years of hiding. An estimated one-third of the global population has latent TB, which accounts for 1.7 million deaths per year—more than any other bacterial pathogen on the planet. 

During the early stages of the disease, active TB bacteria replicate inside human macrophages and dendritic cells. But there is no evidence these cells harbor dormant TB, and the location of the latent reservoir has remained a mystery.

Bikul Das, the study’s first author and a postdoc at Stanford University, first stumbled across a possible reservoir 15 years ago, while working for the World Health Organization (WHO) in the Himalayan country of Bhutan, where TB is severely endemic. While conducting bone marrow biopsies of patients with unexplained pyrexia, or fever, Das became curious as to whether TB could inhabit the bone marrow. Given that active TB can sometimes circulate in the bloodstream, he reasoned, perhaps the bacterium also infects the bone marrow, which is highly vascularized. Sure enough, when he examined surplus biopsy material, Das detected a surprising number of patients that harbored TB in their bone marrow.

Interestingly, much of the TB was observed in progenitor cells, suggesting TB might target bone marrow stem cells, but Das’s tenure in Bhutan ended before he could further explore the finding. Then, 4 years ago, he joined the Stanford University lab of Dean Felsher to revisit the topic. Das and Felsher started by collecting the bone marrow of healthy human volunteers, and exposing the cells in culture to virulent or avirulent strains of TB. The cultures were then treated with an antibiotic to kill any bacteria that hadn’t entered the cells.

Four days later, a subset of bone marrow mesenchymal stem cells (BM-MSCs)—those that expressed the cell surface receptor CD271—carried a remarkably heavy load of TB bacteria. In a follow-up experiment, the researchers tagged TB with a fluorescent marker and watched as the bacteria preferentially infected CD271-positive BM-MSCs. They also checked whether the bacteria could establish latent infections, and found that TB harvested from BM-MSCs up to 2 weeks after initial exposure could successfully grow active cultures.

To confirm the results, Das used a mouse model developed by Antonio Campos-Neto at the Forsyth Institute, where rodents are infected with a genetically modified TB strain whose replication can be switched off after 3 weeks without killing the bacteria. Six months later, Das uncovered viable, nonreplicating TB in the animals’ BM-MSCs. Transferring infected BM-MSCs into tail veins of naïve mice developed severe lung infections, which is indicative of a latent infection in mouse BM-MSCs

Felsher believes that TB might select this hideout because “MSCs express genes that spit out a broad spectrum of drugs,” such that the bacteria would be sheltered from their anti-microbial activity. These stem cells also reside in an immunoprivileged niche within the bone marrow, where multiple mechanisms cooperate to prevent immune attack, such as an elevated density of immunosuppressive Treg cells and higher expression anti-inflammatory molecules.

Interestingly, the researchers found that differentiated BM-MSCs were less supportive of TB infection, with bacterial viability reduced four-fold, as measured by colony-forming assays. These findings raise the question: Does TB compel BM-MSCs to retain their stem cell properties? A recent study in Cell showed that Mycobacterium leprae—the agent of leprosy—reprograms Schwann cells so they adopt stem-cell like properties, which promotes infection.

Finally, the investigators returned to an endemic TB region—Arunachal Pradesh, India—to collect human bone marrow from nine patients who had contracted TB, but had been successfully treated with “DOTS”—a WHO-endorsed, multistep therapy. The researchers isolated CD271-positive BM-MSCs with magnetic sorting, and found that these cells were positive for TB DNA in 8 of the 9 patients. Furthermore, two of the TB-positive bone marrow samples yielded viable bacteria.

While previous studies have detected TB DNA in other cell types, including epithelial/endothelial, fibrocytes, adipocytes, this is the first demonstration of viable TB being isolated from patients with latent infection.

“It’s worth studying how TB gets into mesenchymal stem cells because, in theory, blocking [this interaction] might be a way of reducing dormancy and reoccurrence,” said Felsher.

Das et al., “CD271+ bone marrow mesenchymal stem cells may provide a niche for dormant Mycobacterium tuberculosis,” Science Translational Medicine, 5:170ra13, 2013.

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Comments

Avatar of: sankalp

sankalp

Posts: 1

February 6, 2013

Surface-enhanced laser desorption/ionization (SELDI)TOF assay for the tubercle peptide titre with an assay specific for the macrophages harbouring the bacteria and a parallel Foxp3-sufficient conventional T cells ,nTreg cells reconstituting the i Treg pool for established tolerance with individual concentration of cells in a tuberculin positive patient with and without active disease may be useful for predicting the pathogenesis of a pathogenic mycobacterial sp.

Avatar of: Alexandru

Alexandru

Posts: 70

February 20, 2013

To destroy the all kind of mycobacterium with potentially harmful effects the man must wakeup the Adam mtDNA existed only in xifoid process coupled during ovum fertilization (Genesis) with his Eve mtDNA. Please, logically accept that somebody can wireless write "not on stone tablets but on human hearts" (Paul, 2 Corinthians 3.3 and Hebrew 4.12 and Daniel 7.2-4)

Please, carefully read the Bible, according Daniel 12.4, and my research exposed in "Mitochondrial Adam DNA data transmissions theory" - ISBN 978-606-92107-1-0

Abstract: Brain and soul storming - The necessary and sufficient processes to a well function of the human body are meticulous arranged by specific organizational cells, so called process bio-managers, using interconditioned procedures, transmitted through three ways of communication: chemical or “protein channel”, electrical or “ion channel” and mitochondrial or “EMF wireless channel”. The third type is out of the visible and measurable spectrum and raises a new challenge to the scientist. For this type of bio communication we bring a new theoretical hypothesis, based on the managerial multidisciplinary analysis of a cybernetic model proposed by us, by simulating the human body function with the virtual computerized system based on the management of its total knowledge and its perfect quality way of function. The main bricks used for this virtual construction are: the brain, as main bio-processor, and Eve mtDNA and Adam mtDNA, as bio-antennas. This assembly of the total knowledge, build with “brain reasoning, biological feeling, and unlimited soul feeling”, is called by us “main decision triangle, IQ-EQ-CQ”. The main principle of the management of the total knowledge imposes us to not neglect the information produced by man during the time, even if it seems creasy at the beginning (see brainstorming definition). Because in the natural fertilisation the spermatozoids are naturally equipped with the paternal mtDNA (it looks like reflex klystron power amplifier, KPA = a veritable main bio-GPS), we consider that the paternal mitochondria DNA have a very important role in the evolution of the human being life quality and we have developed a new hypothesis, “Adam mtDNA theory”, in addition to “Eve mtDNA theory”.

Keywords: brain, mitochondria, maternal, paternal

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