Genetic Diversity in the Brain

Neurons within a person’s brain exhibit striking variations in DNA copy number.

By | October 31, 2013

WIKIMEDIA, GARPENHOLMGenomic analyses of single human neurons—either from postmortem brains or those derived in culture—reveal a considerable degree of DNA copy number variation, according to a paper published today (October 31) in Science. It is likely that these genetic differences affect brain cell function, and they may even shape our personalities, academic abilities, and susceptibilities to neurological diseases.

“It’s an exciting paper. It’s a closer look at the single cell genomes of neurons . . .  and it identifies another layer of genomic mosaic changes that are occurring amongst neurons,” said Jerold Chun, a professor of molecular and cellular neuroscience at The Scripps Research Institute in La Jolla, California, who was not involved in the work.

The other genetic changes in neurons, to which Chun referred, are aneuploidy—changes in chromosome number—retrotransposition events—replications of short DNA elements that insert themselves across the genome—and the expression of DNA-altering enzymes, all of which are particularly abundant in the brain.

Ira Hall, a professor of biochemistry and molecular genetics at the University of Virginia who was one of the lead authors on the new study, was interested in whether DNA copy number variations were also prevalent in brain cells. “There was a really long-standing hypothesis that given the huge diversity of cell types in the brain, there might be [genetic] mechanisms . . . to generate [the] diversity,” Hall explained.

But, for about a decade, Hall’s question had gone unanswered. In the early days, “the technology wasn’t really there,” he said. The problem was that if one sequenced the DNA of a given tissue, the readout was an average of all the genomes of a huge population of cells.  “So even if there is lots of variation amongst those cells, you don’t see it,” Hall said. “It became clear that we had to find a way to look at single cells.”

Hall got his wish. Thanks to a single-cell sequencing technique devised recently by scientists at Cold Spring Harbor Laboratory in New York and their colleagues, Hall has been able to sequence the genomes of 110 individual neurons from the postmortem brains of three individuals. He found that a striking 41 percent of the cells contained one or more copy number variations. Most of these were sub-chromosomal alterations—either deletions or duplications.

He and his colleagues also found similar copy number variations in neurons derived in culture from human induced pluripotent stem cells (iPSCs). Fred Gage and colleagues at the Salk Institute for Biological Studies in La Jolla, who collaborated on the paper, studied 40 individual iPSC-derived neurons using a recently developed single-cell microarray-based strategy, and found that 13 of the cells had unique changes to their genomes.

Interestingly, neural progenitor cells derived from the same iPSC lines did not exhibit such abundant diversity. This suggested that the genetic variation in neurons occurred only at later stages of differentiation. And that the variation developed in a short space of time—the seven weeks it took to differentiate neurons from neural progenitors. Furthermore, the results implied that genetic variations seen in adult postmortem brains were unlikely to be a mere side effect of aging.

“It certainly would suggest that some of the changes are occurring pretty early,” said Chun. However, “these changes will stick around for your lifetime, and thus their potential to shape brain function becomes more germane.” Although it is possible that such genetic diversity may occur in some other tissues, he added, it might not have such life-long effects.

Indeed, Chun said it is possible that the observed genetic diversity in the brain may influence behavior, intelligence, and susceptibility to neurological and neuropsychiatric diseases. “I would say it is certain there will be functional consequences for at least some of these changes,” he explained.

The key to answering such questions will be to investigate whether the copy number variations translate to gene expression changes, said Nicholas Navin, a professor of genetics at the MD Anderson Cancer Center in Houston, Texas, who was part of the group that developed the single-cell sequencing technique, but did not participate in the study.  “I think in future work it would be nice to actually have both transcriptome and genome copy number profiles to see how that diversity is affecting expression levels,” he said, noting that technology has yet to allow for such dual-analyses on a single cell.

M.J. McConnell et al., “Mosaic copy number variation in human neurons,” Science, 342: 632-637, 2013.

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Avatar of: somey


Posts: 8

November 1, 2013

So beautiful to go through this article because now the time has arrived to land on the core of the life mystery. Definitely single cell gene sequencing technique has added much impetus with rest of the and  by no means less  processes to unfold the nature's most complicated network.

Avatar of: LeeH


Posts: 35

November 1, 2013

Recent evidence is that aneopoidy precedes and drives cancer.  What implications does this have for neurons or is aneuploidy in neurons an exception?   

Avatar of: Ferez


Posts: 12

November 1, 2013

It is gratifying to know the extent of mammalian genomic instability and epigenetic basis for some of these sequence changes. We showed this with the mouse Y chromosome in 1987:




Avatar of: James V. Kohl

James V. Kohl

Posts: 462

November 1, 2013

The neuronal copy number variation (CNV) can be traced to its origins in yeasts, when epigenetically-effected CNVs enable self vs non-self recognition at the advent of immune system function and sexual reproduction, albeit sans neurons.

Olfactory/pheromonal input is subsequently associated with alternative splicings that link the epigenetic ‘landscape” to the physical landscape of DNA in the organized genome of species from microbes to man via CNVs. During adaptive evolution the CNVs in neurons are directly linked from olfactory/pheromonal input via ecological, social, neurogenic, and socio-cognitive niche construction. For example, neuronal niche construction in nematodes proceeds across species via conserved molecular mechanisms required for the thermodynamics of seemingly futile nutrient-dependent cycles of protein synthesis and degradation to species-specific pheromones. Pheromones control the physiology of reproduction and help to control nutrient-dependent organism-level thermoregulation.

In a mammal, see for example: Odorant receptor gene choice and axonal wiring in mice with deletion mutations in the odorant receptor gene SR1. However, see also my ISHE Summer Institute poster session for details that eliminate mutations from further consideration in adaptive evolution. To do that I used examples from nematodes, insects, other mammals, and a human population that arose in what is now central China during the past ~30,000 years.

Non-random experience-dependent adaptive evolution occurs due to a thermodynamically controlled single base pair change and nutrient-dependent amino acid substitution best exemplified in the mouse model via what is neuroscientifically known about nutrient-dependent pheromone-controlled reproduction.

Avatar of: Roy Niles

Roy Niles

Posts: 109

Replied to a comment from James V. Kohl made on November 1, 2013

November 14, 2013

Baloney.  You have no evidence to support any of this, and are just trying to sell moreof the perfume that you manufacture.  You will of course now cite papers that also had no experimental work detailed that were published in pseudoscientific journals.  You also wrote a book that you cite as independent evidence for the papers that you cite as evidence for the baloney in the book.

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