Quantitative Mycoplasma DNA Materials

New preparations are designed for inclusivity/exclusivity testing, establishing limits of detection, and validating or comparing test methods.

By | April 16, 2014

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ATCC, the premier global biological materials resource and standards organization, announces the expansion of its current solutions for mycoplasma quality control to include quantitative mycoplasma genomic DNA prepared as Certified Reference Materials. These preparations support our wide range of quality control products, which include the Universal Mycoplasma Detection Kit and a collection of 10 titered mycoplasma reference strains. ATCC Certified Reference Materials are calibrated to one or more specified properties, making them ideal for use in challenge assays, verifying or comparing test methods, and benchmarking critical assay performance during assay validation or implementation.

Mycoplasma species are frequent contaminants of cell cultures, affecting numerous phenotypic and functional characteristics of cells. To facilitate the rapid and reliable PCR-based detection of mycoplasma in cell culture, animal sera, and other raw materials, ATCC recently released quantitative mycoplasma DNA Certified Reference Materials. These nucleic acid preparations were isolated from strains most frequently associated with cell culture contamination, and were quantified for genome copy number using digital PCR-based technology.

“The inherent variability of biological materials brings unique challenges to establishing standards for in vitro model systems and to establishing ISO compliant processes to produce them,” said Mindy Goldsborough, Ph.D., VP and General Manager of ATCC Cell Systems in Gaithersburg, Maryland. “By producing Certified Reference Materials under an ISO Guide 34 accredited process, ATCC is able to provide biological materials with confirmed identity, well-defined characteristics, and an established chain of custody. These qualities make ATCC Certified Reference Materials ideal as biological standards for research and development purposes, and provide scientists complete confidence that their results are reliable and reproducible.”

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ATCC Quantitative Mycoplasma DNA

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