Supporting the “Good” Gut Microbes

During systemic infection, mice kick-start the production of a specific sugar to feed and protect the beneficial bacteria in their guts while fighting pathogenic strains.

By | October 1, 2014

Mouse small intestine villi stained for fucose-attached proteins. JOSEPH PICKARD, UNIVERSITY OF CHICAGOMice with systemic bacterial infections induce a pathway that makes a sugar called fucose readily available to feed the beneficial microbiota in the small intestine, according to a study published today (October 1) in Nature. This newly uncovered protective mechanism helps maintain the “good” bacterial populations in the gut while the animal is sick—and appears to protect against further infections.

“The most interesting aspect of this study is that the host is responding to a systemic microbial infection signal by altering glycans on intestinal epithelial cells, and this in turn increases host fitness in a microbiota-dependent manner,” said Laurie Comstock, a microbiologist at the Brigham and Women’s Hospital in Boston who wrote an editorial accompanying the study but was not involved in the work.

In the event of systemic bacterial infection, the host will try to neutralize or kill the harmful bacteria—known as a resistance response—and mitigate the negative impacts of the infection without directly targeting the pathogens—through what’s called a tolerance response. The innate immune system is known to mediate resistance mechanisms to these infections partly by releasing the cytokine IL-22 from innate lymphoid cells. This latest study now shows that IL-22 is also responsible for a tolerance response, by controlling this newly identified fucosylation pathway in the gut in response to the infection.

“This cytokine is known to control anti-microbial defenses,” said study coauthor Joseph Pickard, a graduate student in immunology at the University of Chicago. “Our study adds another, non-bactericide IL-22 function—the modulation of gut microbe activity by providing beneficial gut bacteria with a food source to support them during the infection.”

“Traditionally, studies of host-microbe interactions in animals have focused on the resistance response of detection and killing of microbes,” Janelle Ayres, an assistant professor at the Salk Institute for Biological Studies in La Jolla, California, who studies tolerance defenses in host-microbe interactions, wrote in an e-mail to The Scientist. But according to Ayres, tolerance as a defense strategy has been relatively unexplored in animals. “This elegant study demonstrates that the host employs a fucosylation-based mechanism to maintain a symbiotic relationship with its microbiota during infection, and in doing so, has unraveled a novel tolerance defense strategy,” wrote Ayres.

Along with Alexander Chervonsky, a professor of pathology at the University of Chicago, Pickard and his colleagues first observed inducible fucosylation of proteins in the small intestine upon systemic infection in mice. Ruling out a role of fucosylation in modifying digestive enzymes in the guts of the mice, the researchers tested whether addition of fucose to these proteins was serving as a food source for the microbiota. During a systemic infection, an animal typically stops eating, partly to conserve energy for defense against the pathogen. However, this typically has a detrimental effect on its microbiome.

Although mammalian cells use fucose as a signaling molecule, it is only a food source for microbes. The enzymes of specific microbes in the stomach and the intestines of animals can cleave the fucose attached to proteins. The researchers posited that this might be a way for the host to counteract the negative effects on the gut during systemic infection.

Fucose is available in the stomach and large intestine of healthy animals but is generally absent from the small intestine. The researchers injected molecules—Toll-like receptor ligands—that mimic a systemic infection into pathogen-free mice, resulting in sick animals that consumed less food and water and lost weight. Several hours after the injection, fucose was detectable in small intestine cells in wild-type mice but not those lacking the Fut2 gene, which encodes the (1,2) fucosyltransferase enzyme that fucosylates proteins on these cells. Innate immune cells were enough to induce fucose, the researchers found, as animals lacking T-cells could still induce the addition of the molecule on epithelial small intestine cells.

While pathogen-free Fut2-lacking mice were healthy, those induced with a systemic infection did not recover as well as wild-type mice—they gained weight back slowly. The benefit of fucosylation in the gut seemed to be dependent on the microbiota, as mice with a systemic infection response that were given antibiotics also showed delayed weight gain as they recovered.

Commensal gut bacteria appeared to sense the presence of fucose in the systemically sick mice. These mice, which were also colonized with a reporter strain of E. coli expressing green fluorescence protein (GFP) linked to fucose metabolism genes, showed more GFP expression and higher expression of these genes, dependent on the presence of Fut2.

Wild-type mice with systemic infections that were exposed to a bacterial gut pathogen were able to recover more quickly compared to those without Fut2. Fut2-mutated mice also had higher expression of bacterial virulence genes upon systemic infection compared to wild-type animals. Only the systemic but not the gut bacterial infection resulted in fucosylation, indicating that this mechanism is a response to the systemic bacterial infection, said Chernovsky.

“The next big question is: What is the role of the microbiota in this process?” said Comstock. “Is it that these fucose-harvesting bacteria are able to occupy mucosal niches in the gut that may help protect the host during infection, or is there another mechanism?”

Chervonsky now wants to see how important this fucosylation pathway may be in humans. “About 20 percent of the population is missing the Fut2 gene, and this has been linked to Crohn’s disease.”

“We want to now see whether the lack of fucosylation in humans affects the gene expression in microbes and creates an inflammatory environment in the gut.”

J. Pickard et al., “Rapid fucosylation of intestinal epithelium sustains host–commensal symbiosis in sickness,” Nature, doi:10.1038/nature13823, 2014. 

Add a Comment

Avatar of: You

You

Processing...
Processing...

Sign In with your LabX Media Group Passport to leave a comment

Not a member? Register Now!

LabX Media Group Passport Logo

Comments

Avatar of: WIS

WIS

Posts: 2

October 2, 2014

Phage therapy seems like an obvious way to treat some infections.  But what about the fact that phage are antigenic so that a second dose after another infection might not be effective.  This never seems to be part of articles or discussions on the use of phage in place of antibiotics.

Avatar of: Sue50

Sue50

Posts: 11

October 3, 2014

After working in the ground water business. I wonder how chlorine effects the gut bacteria, especially in the summer when its at its highest level in our water? Growing up on well water you can really compare the difference in your skin, dried out and hair. Maybe these science studies are looking at the result of our environment killing off our good bacteria with the bad. So everyone is now taking probiotics to improve their blood pressure, digestion, etc. maybe we have killed our natural defenses. 

Popular Now

  1. Publishers’ Legal Action Advances Against Sci-Hub
  2. Metabolomics Data Under Scrutiny
    Daily News Metabolomics Data Under Scrutiny

    Out of 25,000 features originally detected by metabolic profiling of E. coli, fewer than 1,000 represent unique metabolites, a study finds.

  3. How Microbes May Influence Our Behavior
  4. Do Microbes Trigger Alzheimer’s Disease?
AAAS