Validate Your Antibodies

An Urgent Need

By | November 1, 2014

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Bethyl Laboratories, Inc. has been dedicated to supporting scientific discovery through its qualified antibody products and custom antibody services since its founding in 1972.

Currently, Bethyl’s portfolio consists of over 7,150 catalog products: close to 5,700 primary antibodies targeting over 2,700 proteins and 1,450 secondary antibodies raised against immunoglobulins from over 25 species.

With over 40 years of experience, Bethyl is also a leading provider of custom antibody production services. Bethyl offers complete packages from initial peptide synthesis to affinity purification of custom antibodies from an antigen-specific immunosorbent.

Every antibody that Bethyl sells has been manufactured to exacting standards at its sole location in Montgomery, Texas, and has been validated in-house by Bethyl’s team of scientists. From the veterinary facilities to the development, production and validation labs, the entire Bethyl team focuses on delivering quality products.

What does it take for antibody to pass Bethyl’s validation and quality control? In other words, what makes a really good antibody? First and foremost, an antibody must be shown to recognize the intended protein target. An antibody must also show high sensitivity with minimal cross-reactivity. To achieve these goals, Bethyl has devised a unique process for validating the specificity of its antibody products. While many companies settle on using data from a single antibody, Bethyl utilizes a reciprocal testing system that incorporates multiple antibodies raised to different regions of the same protein target.

Only after an antibody has been validated for specificity in immunoprecipitation and/or Western blot is it then tested in additional applications such as immunohistochemistry, immunocytochemistry, ChIP, and proximity ligation assay.

Because of its rigorous validation process and high standards, Bethyl does not sell every antibody it makes. Bethyl serves to advance science by concentrating its resources on developing qualified antibodies, including many to emerging and underserved protein targets.

To learn more about Bethyl’s validation process and its products, please visit www.bethyl.com.

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