Probing Down Syndrome with Mini Brains

Researchers create cerebral organoids using induced pluripotent stem cells from patient skin cells and characterize protein-expression changes linked to cognitive impairment.

By | October 20, 2015

WIKIMEDIA, NATIONAL HUMAN GENOME RESEARCH INSTITUTEInduced pluripotent stem cells (iPSCs) grown from the skin cells of a person with Down syndrome are helping researchers grow cerebral organoids and track protein expression in an effort to better understand the disorder on a cellular and molecular level. University of Colorado Boulder postdoc Tristan McClure-Begley and his colleagues sought to better understand the neurological and developmental changes that occur in people with a third copy of chromosome 21, the unifying pathological feature of Down syndrome. “That third copy of chromosome 21 influences all aspects of embryonic development, including critical steps during brain development,” McClure-Begley said in a statement. “But we’ve had trouble identifying exactly why the extra chromosome has such widespread effects, partly because we’ve lacked good human tissue models of Down syndrome.”

So McClure-Begley and his colleagues took a fibroblast from a single Down syndrome patient, coaxed it into becoming two iPSC lines, and then reprogrammed those cells into neural progenitor cells that self-organized into cerebral tissue in vitro. “So we get a window into what an individual central nervous system development looked like from a cellular and molecular level,” he said during a Monday (October 19) press conference at the Society for Neuroscience (SfN) annual meeting held in Chicago.

The researchers found that protein expression did not follow a simple 1:1 ratio with the extra genes on the triplicate chromosome 21 leading to a proportional increase in proteins expressed. “Rather, there’s a complex change, where some proteins are upregulated, some proteins are downregulated—all reflecting a general disturbance in signaling,” McClure-Begley said. Many of the perturbed proteins were involved in early neurogenesis and cell cycle regulation, among other functions.

Earlier this year, researchers in Germany reported similar success growing cerebral organoids from iPSCs derived from skin cells of people with autism.

McClure-Begley added that his group plans to generate as many organoids from Down syndrome patients as possible in order characterize protein-expression profiles across the spectrum of the disorder, from mild to severe. “We can start observing . . . the discrete factors in their genome not on chromosome 21 that can influence the severity of their clinical presentation of Down syndrome,” he said. “By doing that, it’s our hope that we will actually be capable of then correlating clinical severity with a molecular signature from these tissues, thereby informing our ability to come up with tailored, personalized treatments to improve quality of life.”

Add a Comment

Avatar of: You



Sign In with your LabX Media Group Passport to leave a comment

Not a member? Register Now!

LabX Media Group Passport Logo

Popular Now

  1. A Potential Remedy for the Aging Brain
    The Scientist A Potential Remedy for the Aging Brain

    In mice, injected fragments of a naturally occurring protein boost memory in young and old animals and improve cognition and mobility in a model of neurodegenerative disease. 

  2. The Sleeping Brain Can Learn
    Daily News The Sleeping Brain Can Learn

    Humans can remember new sensory information presented during REM sleep, but this ability is suppressed during deep, slow-wave slumber.

  3. USDA Emails: Don’t Use “Climate Change”
  4. Nature Index Identifies Top Contributors to Innovation