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Spinning Straw into Gold
The Scientist 2004, 18(1):40
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X-ray crystallography generate selegant, yet static, portraits for biologists' ever-growing gallery of protein structures. To reveal protein motion, bio-physicists have developed site-directed spin labeling (SDSL), which uses electron paramagnetic resonance (EPR) spectroscopy to probe protein dynamics. The technique, performed using an EPR spectrometer, is complementary to nuclear magnetic resonance (NMR) but also offers some distinct advantages, including higher sensitivity, applicability to larger proteins, and the potential for real-time measurements.
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