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Consider the Cycler
From thermocyclers to thermostable polymerases, PCR buyers have options
The Scientist 2004, 18(17):34
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Earlier this summer scientists from New England Biolabs in Beverly, Mass., announced they had discovered a new way to copy DNA. Their method, called helicase-dependent amplification (HDA), reportedly mimics in vivo DNA replication better than PCR does.[1]
Moreover, HDA can be conducted at a single temperature, thus obviating the need for thermocycling. In the future, perhaps the limitations of PCR – that it is reagent intensive, requires expensive equipment, is difficult to reproduce, and generally is not linear – will be surmounted by new technologies, HDA or otherwise.
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