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mRNA Amplification, sans PCR
NuGEN's Ribo-SPIA technology uses DNA/RNA hybrids to amplify transcripts without thermal cycling
The Scientist 2004, 18(19):36
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By putting two enzymes on the same shift, San Carlos, Calif.-based NuGEN http://www.nugeninc.com can turn five nanograms of total RNA into enough cDNA for a microarray in just four hours. Key to the technology, termed Ribo-SPIA, are minimal starting material, speed, and linearity, says Anne Kopf-Sill, vice president of product development.
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