Advertisement
Survey Series
For Advertisers
|
|
||||
|
Scratching the Cell Surface
Total internal reflection fluorescence microscopy exposes events at the cell membrane
The Scientist 2004, 18(22):27
|
||||
|
|
||||
|
Most biological microscopes delve deep into the cell, imaging optical slices that can be put together into a three-dimensional rendering of what lies beneath the cell membrane. But a lot of biology takes place at the cell surface. Vesicles and receptors cycle between the membrane and the cytoplasm, propagating cellular signals by way of transient interactions between cellular proteins, macromolecular assemblies, and organelles. To catch a glimpse of these events, a growing number of researchers use total internal reflection fluorescence (TIRF) microscopy, also known as evanescent wave microscopy.
|
|
|||||||||
|
|||||||||
|
