PNNL Team Captures Individual Protein Interactions :The Scientist [22/11/2004]

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by Aileen Constans and David Secko

TOOLS AND TECHNOLOGY

PNNL Team Captures Individual Protein Interactions

Single-molecule photon-stamping spectroscopy coupled with a fluorescent biosensor detects protein motions in real time

The Scientist 2004, 18(22):34

Published

22 November 2004

Scientists at the Pacific Northwest National Laboratory in Richland, Wash., have used an established technique to observe real-time interactions between single protein molecules for the first time. Ultimately, the PNNL method – single-molecule photon stamping spectroscopy – will be used to study signaling events in living cells. At present it offers more information about protein-protein interaction dynamics than can be obtained with conventional structural biology techniques such as nuclear magnetic resonance (NMR) and X-ray crystallography.


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